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Determination method of lycopene

A determination method and technology of lycopene, which is applied in the field of food additives and its detection, can solve the problems of not accurately reflecting the content of lycopene

Inactive Publication Date: 2015-01-28
西安莹朴生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The content of lycopene in tomato and its products such as tomato paste and tomato oleoresin is mainly determined by spectrophotometry, but due to the absorption of other pigments such as β-carotene, the results cannot accurately reflect the content of lycopene

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Standard curve creation:

[0014] After dissolving 1mg of the standard product in mobile phase, dilute to 50mL and prepare a 20μg / mL standard solution. ; flow rate 0.9ml / min; column temperature at room temperature 25°C; detection wavelength 472nm), inject 20 μL of sample, perform regression on concentration X with relative peak area Y, and calculate the regression equation. The relationship curve equation is:

[0015] Y=2.0842X+2.0043, r=0.9907, good linear relationship.

[0016] (2) Sample determination:

[0017] Accurately weigh 0.2g sample, first add a small amount of dichloromethane to dissolve, then use mobile phase to make the volume to 200mL, inject 20μL sample under the same chromatographic conditions, and calculate the concentration according to the obtained relative peak area.

[0018] According to the determination method of the invention, the recovery rate range of lycopene is 90.55-105.48, which meets the analysis requirements, and each detection re...

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Abstract

The invention relates to a determination method of lycopene, and belongs to the field of food additives and detection methods of the food additives. The determination method of the lycopene comprises the following steps: dissolving 1mg of standard substances by using a mobile phase, making volume up to 50mL, preparing a 20microg / mL standard solution, feeding 20microL of a sample under certain chromatographic conditions, regressing the concentration X by a corresponding peak area Y, and calculating to obtain a regression equation; and accurately weighing 0.2g of the sample, firstly adding a small amount of dichloromethane for dissolving, then making volume up to 200mL by using the mobile phase, feeding 20microL of the sample under the certain chromatographic conditions, and calculating the concentration according to the obtained corresponding peak area. The determination method determines the content of the lycopene by adopting an HPLC method, has the advantages of simplicity, convenience, accuracy, reliability and the like, and is suitable for being used in common laboratories.

Description

technical field [0001] The invention relates to a method for determining lycopene, which belongs to the field of food additives and detection methods thereof. Background technique [0002] Lycopene is a very important carotenoid with superior physiological functions. It has a strong ability to scavenge free radicals and quench singlet oxygen. Its rate constant for scavenging singlet oxygen is 100 times that of vitamin E, a commonly used antioxidant, and twice that of β-carotene. Recent epidemiological studies have shown that eating more foods rich in lycopene can greatly reduce the risk of certain cancers in the human body. Lycopene is increasingly recognized as being negatively associated with cancer risk together with other carotenoids. related, and even the only carotenoids negatively associated with certain cancers. Now more and more studies show that lycopene can prevent the occurrence of prostate cancer, breast cancer and digestive tract (including colon, rectum and...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 李勇刘文斌
Owner 西安莹朴生物科技股份有限公司
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