Method for preparing swine O-type foot-and-mouth disease synthetic peptide antigen 2800 by solid-phase fragment method

Abstract

The invention provides a method for preparing pig O-type foot-and-mouth disease synthetic peptide antigen 2800 by using a solid-phase fragment method. The method comprises the following steps: by taking resin as an initial raw material, sequentially connecting amino acid with 9-fluorene methoxycarbonyl group protection, preparing a protected peptide fragment, sequentially removing 9-fluorene methoxycarbonyl groups in the period, performing peptide connection reaction by using a condensating agent, and cutting by using diluted acid or weak acid so as to obtain the protected peptide fragment; gradually connecting the fragment with 4-(4'-dimethoxy-9-fluorene methoxyamine methyl)-phenoxymethyl resin, and further connecting with T-auxiliary cell epitope; cutting off the protection groups and the resin by using acid so as to obtain synthetic peptide antigen 2800 coarse peptide; purifying by using ion exchange and a tangential flow membrane coating system, and concentrating to remove micromolecules and salt, thereby obtaining the synthetic peptide antigen 2800. The method provided by the invention has the characteristics of being stable in process, short in production period, convenient in obtaining raw / auxiliary materials, small in waste, low in production cost, high yield and the like.

Description

technical field [0001] The invention relates to a method for preparing swine type O foot-and-mouth disease synthetic peptide antigen 2800 by a solid-phase fragment method. Background technique [0002] The structural formula of swine type O foot-and-mouth disease synthetic peptide antigen 2800 is as follows: [0003] [0004] Pig foot-and-mouth disease is an acute, febrile, highly contagious infectious disease caused by foot-and-mouth disease virus. In recent years, foot-and-mouth disease has broken out in a large area in Jiangsu, Sichuan, the three northeast provinces and Inner Mongolia, causing huge economic losses. Currently, immunization remains the main means of controlling the disease. The traditional inactivated vaccine is still the leading method for the prevention and treatment of foot-and-mouth disease at home and abroad, but it is easy to cause side effects such as allergies, and there are also problems in biosafety. Therefore, a lot of research has been don...

AI Technical Summary

Problems solved by technology

Although a lot of research has been done on most of the possible side reactions and their mechanisms in the past few years, the yield of a single-step cycle still cannot reach more than 99.5%, and the remaining 0.5% of each step accumulates as by-products of polluting products.

Therefore, the target peptide is easily contaminated by a series of compounds with similar structures and chemical properties, such as diastereoisomers formed by epimerization, wrong sequences and incomplete core sequences, etc.

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