Electrochemical transducer, and preparation method and application for detecting pathogenic bacteria
A pathogenic bacteria and sensor technology, applied in the field of electrochemical sensors for detecting pathogenic bacteria, can solve problems such as time-consuming, time-consuming environmental requirements, and tediousness, and achieve the effects of improving detection sensitivity, easy popularization and application, and simple popularization and application
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0034] Embodiment 1 detects the electrochemical sensor of Campylobacter jejuni
[0035] (1) Preparation of working solution 1
[0036] Working solution 1 is nano-magnetic bead dispersion labeled with anti-Campylobacter jejuni monoclonal antibody, the concentration of nano-magnetic beads is 10mmol / L, and the molar ratio of nano-magnetic beads to anti-Campylobacter jejuni monoclonal antibody is 1:6.
[0037] Dissolve 1.08 g of ferric chloride and 0.2 g of trisodium citrate in 20 mL of ethylene glycol, then add 1.2 g of sodium acetate, stir for 30 min, and place it in an oil bath at 200°C for 10 hours to obtain a concentration of 10 mM nano magnetic bead dispersion.
[0038] Take 60 μL of 10 mM nanomagnetic bead solution, 15 μL of 0.1 mM 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) solution and 0.01 mM N-hydroxy 15 μL of succinimide (NHS) solution, mixed, and reacted at room temperature for 10-30 minutes to activate the nano-magnetic beads; add 0.01 mg anti...
Embodiment 2
[0053] Example 2 Detection of Campylobacter jejuni in actual samples
[0054] 1. Establish a standard curve
[0055] The electrochemical sensor for detecting Campylobacter jejuni in Example 1 was used to detect Campylobacter jejuni. The detection process is at room temperature.
[0056] (1) Take 20 μL of working solution 1 and add it to 1 mL of milk solution supplemented with different concentrations of Campylobacter jejuni (ATCC33560, purchased from Beijing Zhongyuan Company). After mixing, place it on a rotary mixer. 10 minutes. The nanomagnetic beads-Campylobacter jejuni complex was magnetically separated by a magnetic stand, the supernatant was removed, 1 mL of 10 mM PBS (pH 7.4) solution was added, and washed on a rotary mixer for 3 minutes, and then repeated washing 3 times. Finally, 100 μL of PBS buffer (10 mM, pH 7.4) was added to resuspend to obtain the nanomagnetic beads-Campylobacter jejuni complex dispersion.
[0057] (2) Mix 20 μL immunomagnetic beads-Campylob...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com