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Stem cell micro-needle patch for resisting wrinkles and removing freckles and preparation method thereof

An anti-wrinkle and freckle-removing technology, stem cell technology, applied in the direction of medical preparations containing active ingredients, sheet delivery, pharmaceutical formulas, etc., can solve side effects, pain and bruises, treat the symptoms but not the root cause, complications, etc., to promote absorption, youthful skin, improve the effect of intermediary metabolism

Inactive Publication Date: 2015-01-21
奥思达干细胞有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Currently, cosmeceuticals and cosmetology are both temporary measures that treat symptoms but not the root cause, with high risks, complications, side effects, and associated pain and bruising

Method used

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  • Stem cell micro-needle patch for resisting wrinkles and removing freckles and preparation method thereof
  • Stem cell micro-needle patch for resisting wrinkles and removing freckles and preparation method thereof
  • Stem cell micro-needle patch for resisting wrinkles and removing freckles and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Preparation of Stem Cell Microneedle Patch

[0037] 1. The microneedle array made of silicon or metal materials is made by microelectronics manufacturing technology or micro-casting technology, and is sterilized for use.

[0038] 2, prepare stem cell suspension, the stem cell that the present invention contains is adipose stem cell, and the preparation extraction step of adipose stem cell is as follows:

[0039] The first step is to obtain human adipose tissue under sterile conditions, shred it and wash it with PBS solution to remove blood cells;

[0040] In the second step, the digestive juice (containing 0.1% Type collagenase and 2.5% trypsin, prepared by mixing at a ratio of 1:1) to digest adipose tissue, cut it repeatedly until it becomes a paste, and then shake and digest at 170r / min for 30 minutes;

[0041] The third step is to absorb the liquid of the single cells in the lower layer, transfer it into the complete medium (high glucose DMEM containing ...

Embodiment 2

[0048] Example 2 Identification of Adipose Stem Cells

[0049] 1. Drawing of growth curve

[0050] Take well-grown P3 generation cells, digest them with 0.25% trypsin (containing 0.2% EDTA), and dilute them with 2×10 4 / ml concentration inoculated in 24-well plate, 1 mL per well. The OD value of the cells was detected at 0, 24, 48, 72, 96, 120, 144, and 168 hours after planting. Method: Add 20 μL of MTT to each well, place in a 37°C, 5% CO2 incubator and continue to incubate for 4 hours, collect the cells in the well by centrifugation, add 150 μL of DMSO to each well, shake for 10 minutes, and use a microplate reader at 490 nm The OD value is automatically detected at the wavelength. Experiments were repeated three times. After the statistical analysis, take the culture time as the horizontal axis and the OD value as the vertical axis to draw the growth curve, such as figure 2 The growth curve of adipose stem cells shows that the cell proliferation is in good condition a...

Embodiment 3

[0053] Embodiment three face VISIA skin test effect, take the following scheme:

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Abstract

The invention discloses a stem cell micro-needle patch for resisting wrinkles and removing freckles and a preparation method thereof, and particularly relates to a micro-needle transdermal patch which is prepared of a anti-wrinkle and freckle removing essence and is injected into subcutaneous tissues so as to achieve cosmetic results, wherein the anti-wrinkle and freckle removing essence is prepared from stem cells, hyaluronic acid, epidermal growth factors, vitamin E and the like. The micro-needle patch has the advantages that the anti-wrinkle and freckle removing essence is introduced to a corium layer of skin by a microtubule, active ingredients permeate in the skin effectively and efficiently so that skin cells directly absorb all of the active ingredients in the anti-wrinkle and freckle removing essence, good effects are achieved, and finally the skin is vitalized from inside to outside so as to thoroughly relieve wrinkles and color spots from the skin. The stem cell micro-needle patch is used for resisting wrinkles and removing freckles and is safe, effective and fundamentally free of wounds.

Description

technical field [0001] The present invention relates to a stem cell microneedle patch for anti-wrinkle and anti-freckle and its preparation method, in particular to an anti-wrinkle and anti-freckle essence prepared by using stem cells, hyaluronic acid, epidermal growth factor and vitamin E, etc. The skin patch is injected into the subcutaneous tissue to achieve the beauty effect, and belongs to the field of regenerative medicine technology and anti-aging beauty service. Background technique [0002] The cells of the body are always in the process of new cells replacing aging and degenerated cells. When the cell renewal rate is lower than the cell aging rate, there will be insufficient renewal, and signs of aging will begin to appear. The formation of wrinkles and stains on the facial skin is an important feature and indicator . The cells that can regenerate in the body are stem cells, that is to say, the source of skin aging is the lack of stem cell renewal ability. Clinic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/70A61K38/18A61P17/00A61M37/00A61K31/728A61K35/28A61K31/355
Inventor 周萱
Owner 奥思达干细胞有限公司
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