Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of spore powder of yellow basket-shaped fungus, wettable powder of yellow basket-shaped fungus and preparation method thereof

The technology of a yellow tallow bacteria and a wettable powder is applied to a microbial pesticide wettable powder and its preparation, a yellow tallow bacteria spore powder is fermented and prepared, the yellow tall bacteria wettable powder and the preparation field thereof can solve the restriction The use and promotion of Talaromyces yellows has the effect of accelerating the promotion and application process.

Active Publication Date: 2016-08-24
QINGDAO AGRI UNIV
View PDF5 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Talaromyces flavus is one of the important bio-control bacteria. At present, there is no corresponding preparation research and development of this fungus in China, which greatly restricts the use and promotion of Talaromyces flavus in biological control.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of spore powder of yellow basket-shaped fungus, wettable powder of yellow basket-shaped fungus and preparation method thereof
  • A kind of preparation method of spore powder of yellow basket-shaped fungus, wettable powder of yellow basket-shaped fungus and preparation method thereof
  • A kind of preparation method of spore powder of yellow basket-shaped fungus, wettable powder of yellow basket-shaped fungus and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Conditions for Solid Fermentation of Basilisk Yellow Fungus qw12

[0027] 1. Determine the solid fermentation substrate

[0028] Set up three single-substance culture media: bran, corn stalk powder, and corn flour; four mixed-substance culture media: bran+corn stalk powder, bran+corn meal, corn stalk powder+corn meal, bran + corn stalk powder + corn flour, the different ingredients in the mixed substance medium are mixed in equal proportions. Ratio of material to water = 1:1, sterilized at 121°C for 30 minutes, inoculated with 1×10 6 spore suspension per mL, the inoculum size was 5%; cultured at 28°C for 7 days, and turned over once a day. Three replicates were set up for each treatment. After the fermentation, take 0.5 g of the solid fermentation culture, add sterile water, stir on a magnetic stirrer for 5 min, and count the number of spores under a microscope with a hemocytometer. The results show that when the medium is a single substrate, the effect of bran is t...

Embodiment 2

[0075] Embodiment 2: the preparation of yellow basket-shaped fungus spore powder

[0076] The optimized spore-forming medium of the yellow basket-shaped bacteria was sterilized at 121°C for 20-30 minutes and inoculated for 10 6 Individual / mL spore suspension, cultivated in the dark at 28°C for 10 days. After the fermentation is over, dry the solid medium in an oven at 35°C to dry the water, dry and crush the solid culture and pass it through a 200-mesh sieve; or use a fungal spore separator to separate the spores and pass through a 200-mesh sieve; the collected yellow basket-shaped fungus Conidia powder.

[0077] Weigh 0.05g of the spore powder of the yellow basket-shaped fungus and suspend it in 20mL of sterile water, stir it with a magnetic stirrer for 30 minutes, then use a hemocytometer to measure the concentration of the spore suspension, and calculate the spore content of the yellow basket-shaped fungus conidia powder. Repeat 3 times to calculate the average spore cont...

Embodiment 3

[0081] Embodiment 3: the screening of auxiliary agent

[0082] 1 Carrier screening

[0083] The effect of carrier on the germination of spores of T. jaundice: Kaolin, talcum powder, bentonite, and diatomaceous earth are mixed with PDA medium at a concentration ratio of 5 mg / mL and 10 mg / mL, sterilized, and prepared under sterile conditions flat. Take 1.0×10 3 0.1mL of spore suspension of T. yellow qw12 per ml was spread on plates containing different carriers. The PDA medium without carrier was used as a control. Each treatment was repeated 4 times, cultured at 28°C, and observed after 48 hours. Count the number of colonies formed by spore germination on plates containing the different carriers.

[0084] The influence of the carrier on the mycelial growth of the yellow basket-shaped fungus: put the yellow basket-shaped fungus qw12 bacterial block with a diameter of 0.5cm in the center of the plate containing different carriers made by the above method, and use the carrier-f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
suspension rateaaaaaaaaaa
Login to View More

Abstract

The invention relates to a Talaromyces flavus wettable pulvis. The Talaromyces flavus wettable pulvis includes Talaromyces flavus conidiospore powder, a carrier and an assistant, the content of living bacteria in the Talaromyces flavus wettable pulvis is 5*10<8>-5*l0<9>cfu / g / g, the suspension rate of the Talaromyces flavus wettable pulvis is greater than 75% wettable powder, the wetting time is less than 60s, and the water content is less than 6%. In the invention, the spore fermentation technology of Talaromyces flavus aw12 strains is optimized; and the Talaromyces flavus wettable pulvis is created by using Talaromyces flavus spores as an active component. The invention provides a production method of a Talaromyces flavus biocontrol preparation, and a new convenient and practical dosage form. The Talaromyces flavus spore powder preparation method lays a foundation for the large scale commercialization production of the Talaromyces flavus wettable pulvis, is in favor accelerating the application process of Talaromyces flavus in the biological control, and is of theoretic and practical significance.

Description

technical field [0001] The present invention relates to the technical field of microbial pesticides, in particular to a wettable powder of microbial pesticides and a preparation method thereof, more specifically, the present invention relates to a method for fermenting and preparing spore powder of yellow basket-shaped fungus, and the yellow basket-shaped fungus can be Wet powder and its preparation method. Background technique [0002] Pesticides play an important role in preventing and controlling plant diseases and insect pests, ensuring stable and increased agricultural production, improving the quality of agricultural products, and improving people's living standards. Chemical pesticides have played an important role in controlling crop diseases and protecting agricultural safety, but they have also caused a series of environmental and social problems, such as the "3R" problem caused by the widespread use of chemical pesticides, etc., seriously affecting the sustainabil...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/04A01P3/00C12N3/00C12R1/645
Inventor 咸洪泉张军霞李雅华
Owner QINGDAO AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com