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Rapid propagation method of hedyotis caudatifolia calluses and suspension cells

A technology of callus and flag leaf ear grass, applied in the field of plants, can solve the problem of lack of research on propagation and introduction technology, and achieve the effects of less energy consumption, high proliferation rate and simple operation.

Inactive Publication Date: 2015-01-07
NANJING TONGZE AGRI SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the utilization of sword ear grass mainly relies on natural reproduction, and there is no research on its breeding and introduction technology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0009] Take the blades of sword ear grass, soak them in bleaching powder for 3 minutes, remove the surface stains with a brush, wash them with running water for 30 minutes, treat them with sodium hypochlorite on the ultra-clean bench for 11 minutes, rinse them with sterile water 5-7 times, and sterilize the sword leaves The formula of the eargrass leaf insertion medium is MS+IBA0.5mg / L+6-BA4mg / l+activated carbon 0.1% medium for callus induction culture, additional sucrose 30g / L, agar 6.5g / L, PH5-6 , cultured in a dark room at a temperature of 20°C, and the induced callus with good dispersion was put into liquid medium B 5 +NAA0.1mg / L+KT0.2mg / L+casein hydrolyzed amino acid 0.5g / L+folic acid for shaking culture, add sucrose 30g / L, inoculate 50ml liquid suspension cells per 200ml Erlenmeyer flask, pH5.85, light 1000lx, temperature Stable cells cultured in suspension at 20°C were cultured in the above-mentioned liquid medium supplemented with 3 mg / L lanthanum nitrate, weighed afte...

Embodiment 2

[0011] Take the blades of sword ear grass, soak them in bleaching powder for 3 minutes, remove the surface stains with a brush, wash them with running water for 30 minutes, treat them with sodium hypochlorite on the ultra-clean bench for 11 minutes, rinse them with sterile water 5-7 times, and sterilize the sword leaves The formula of the eargrass leaf insertion medium is MS+IBA0.5mg / L+6-BA4mg / l+activated carbon 0.1% medium for callus induction culture, additional sucrose 30g / L, agar 6.5g / L, PH5-6 , cultured in a dark room at a temperature of 20°C, and the induced callus with good dispersion was put into liquid medium B 5 +NAA0.2mg / L+KT0.4mg / L+casein hydrolyzed amino acid 0.5g / L+folic acid for shaking culture, add sucrose 30g / L, inoculate 50ml liquid suspension cells per 200ml Erlenmeyer flask, pH5.85, light 1000lx, temperature Stable cells cultured in suspension at 20°C were cultured in the above-mentioned liquid medium supplemented with 5 mg / L lanthanum nitrate, and weighed ...

Embodiment 3

[0013] Take the blades of sword ear grass, soak them in bleaching powder for 3 minutes, remove the surface stains with a brush, wash them with running water for 30 minutes, treat them with sodium hypochlorite on the ultra-clean bench for 11 minutes, rinse them with sterile water 5-7 times, and sterilize the sword leaves The formula of the eargrass leaf insertion medium is MS+IBA0.5mg / L+6-BA4mg / l+activated carbon 0.1% medium for callus induction culture, additional sucrose 30g / L, agar 6.5g / L, PH5-6 , cultured in a dark room at a temperature of 20°C, and the induced callus with good dispersion was put into liquid medium B 5 +NAA0.2mg / L+KT0.2mg / L+casein hydrolyzed amino acid 0.5g / L+folic acid for shaking culture, add sucrose 30g / L, inoculate 50ml liquid suspension cells in each 200ml Erlenmeyer flask, pH5.85, light 1000lx, temperature Stable cells cultured in suspension at 20°C were cultured in the above-mentioned liquid medium supplemented with 3 mg / L lanthanum nitrate, weighed ...

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PUM

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Abstract

The invention provides a rapid propagation method of hedyotis caudatifolia calluses and suspension cells. The rapid propagation method comprises the steps of obtaining sterile blades, inducing the calluses, culturing and propagating the suspension cells and the like. According to the method provided by the invention, large-scale culture of hedyotis caudatifolia cells can be realized, secondary metabolites are produced, the shortcomings that synthesis by a chemical method is not economic and is affected by natural conditions and human conditions and the like are overcome, and the method further has the advantages of simplicity in operation, good test repeatability and high proliferation rate.

Description

technical field [0001] The present invention relates to the culture of suspension cells of Aurantium saponifera under the condition of tissue culture, and belongs to the technical field of plants. Background technique [0002] sword ear grass, Hedyotis caudatifolia Merr.et Metcalf, Rubiaceae, shrub, alias: long-tailed ear grass, juvenile red, millennium tea, tuberculosis grass, etc., produced in Guangdong, Guangxi, Fujian, Jiangxi, Zhejiang (southern), Hunan and other provinces and regions; common in jungles It can be found on dry sandy soil or cliff walls, and sometimes on grasslands with clayey soils. Sword leaf ear grass is a kind of medicinal plant, the whole plant: sweet in taste and flat in nature. It has the functions of relieving cough, eliminating stagnation and hemostasis. For bronchitis asthma, tuberculosis, hemoptysis, infantile malnutrition. Fresh leaves: pounded and applied externally on bruises, swelling and pain, traumatic bleeding. Leaves: decoction an...

Claims

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Application Information

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IPC IPC(8): C12N5/04C12N5/02
Inventor 杨存
Owner NANJING TONGZE AGRI SCI & TECH
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