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Culture medium and culture method of Irpex lacteus

A kind of technology of white sac bacteria and culture medium, applied in the field of microbial fermentation, can solve the problems of difficult to meet the raw material demand of "Yishenkang", low yield of fermentation process, high production cost, etc., and achieve good activation, high content, and mycelium growth quick effect

Pending Publication Date: 2021-06-11
CHANGCHUN UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in recent years, it has been shrinking, mainly because the original fermentation process has low output and high production cost, which makes it difficult to meet the raw material demand of "Yishenkang", and the large-scale production of enterprises is facing difficulties.
The aging of the bacteria is an important reason for this problem

Method used

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  • Culture medium and culture method of Irpex lacteus
  • Culture medium and culture method of Irpex lacteus
  • Culture medium and culture method of Irpex lacteus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0061] The production of embodiment 1 seed

[0062] (1) Inclined bacteria:

[0063] Medium: PDA medium glucose 2%, potato 20%, agar 2%, pH natural.

[0064] Inoculate the Rake-tooth albicans strain into PDA medium, culture at 26°C-28°C, and mycelia grow all over the slope within 70 hours, and store it in the refrigerator (2-4°C) for later use.

[0065] (2) secondary shake flask strains

[0066] Medium: Glucose 3%, Peptone 2%, KH2PO4 0.2%, MgSO 4 0.1%, natural pH. Sterilized for later use.

[0067] Take the slant strains with short bacterial age and thick and dense hyphae, and insert them into the sterilized shake flask culture medium (according to the ratio of 1:6, that is, 1 slant connects 6 shake flasks) under aseptic conditions, at 23°C ~25°C temperature-controlled culture, shaking flask rotation speed 160-220r / min (rotary type), when the fermentation liquid per 100ml obtains a mycelium dry weight of more than 1.2g; microscopic examination reaches the standard under t...

Embodiment 2

[0071] The production of embodiment 2 seeds

[0072] (1) Inclined bacteria:

[0073] Medium: PDA medium glucose 2%, potato 20%, agar 2%, pH natural.

[0074] Inoculate the Rake-tooth albicans bacteria into PDA medium, culture at 23°C-28°C, and mycelia cover the slope within 71 hours, and store in the refrigerator (2-4°C) for later use.

[0075] (2) secondary shake flask strains

[0076] Medium: glucose 3%, peptone 1%, KH 2 PO 4 0.2%, MgSO 4 0.15%, natural pH. Sterilized for later use.

[0077] Take the slant strains with short bacterial age and thick and dense hyphae, and insert them into the sterilized shake flask culture medium (according to the ratio of 1:6, that is, 1 slant connects 6 shake flasks) under aseptic conditions, at 23°C Temperature-controlled culture at ~28°C, shaker flask rotation speed 160-220r / min (rotary type), when the fermentation liquid per 100ml obtains a mycelium dry weight of more than 0.9g; microscopic examination reaches the standard under t...

Embodiment 3

[0081] The production of embodiment 3 seeds

[0082] (1) Inclined bacteria:

[0083] Medium: PDA medium glucose 2%, potato 20%, agar 2%, pH natural.

[0084] Inoculate the Rake-tooth albicans bacteria into PDA medium, culture at 25°C-28°C, and mycelium cover the slope within 72 hours, and store in the refrigerator (2-4°C) for later use.

[0085] (2) secondary shake flask strains

[0086] Medium: glucose 3%, yeast powder 2%, KH 2 PO 4 0.2%, MgSO 4 0.15%, natural pH. Sterilized for later use.

[0087] Take the slant strains with short bacterial age and strong and dense hyphae, and insert them into the sterilized shake flask culture medium (according to the ratio of 1:6, that is, 1 slant connects 6 shake flasks) under aseptic conditions, at 22°C ~25°C temperature-controlled culture, shaking flask rotation speed 160-220r / min (rotary type), when the fermentation liquid per 100ml obtains a mycelium dry weight of more than 0.9g; microscopic examination reaches the standard u...

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Abstract

The invention relates to the technical field of microbial fermentation, in particular to a culture medium and culture method of Irpex lacteus. The co-growth and development rule of organisms is from a juvenile stage to a vigorous development stage to a senescent stage to a decline stage. According to the fungal biological fermentation engineering principle, a fermentation method is applied, continuous reproduction of hyphae and accumulation of metabolites are promoted, effective active ingredients are extracted, and reliable raw materials are provided for pharmacy and development of other related products. Excellent culture medium matching is the basis of strain growth and development, and is the important key technical problem for fermentation production.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a culture medium and a culture method of Racillus albicans. Background technique [0002] Irpexlacteus Fr. is a kind of fungus of Irpexlacteus Fr., which belongs to the family Irpexlacteaceae, the genus Irpexlacteus, and the species of Irpexlacteus, also known as white sac hole. Synonyms: Hirschioporus lacteus (Fr.) Teng; Deng Shuqun, Fungi in China, 484.1963. The fruiting body of the white rake-tooth fungus is born on the dead standing or fallen wood of broad-leaved trees. Morphological characteristics: Basidiocarp (fruiting body) flattened, expanded, white, soft leathery; or flattened and rolled up, the rolled part is 8-15×10-20mm. Sometimes anatropous, stacked in the shape of compound tiles, fan-shaped, with a narrow base, 2-4cm in transverse diameter, and 2-3mm in original; cover surface is milky white, with short hairs, silky luster, ring pattern, thin edge,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G18/20A01G18/40A23L31/00A23L33/00A23L2/38A61K8/9728A61K36/07A61P7/10A61P9/12A61P29/00A61P37/04A61Q19/00
CPCA01G18/20A01G18/40A23L31/00A23L33/00A23L2/38A61K36/07A61K8/9728A61P7/10A61P29/00A61P9/12A61P37/04A61Q19/00A23V2002/00A23V2200/30A23V2250/208A23V2250/6418A23V2300/10A23V2250/032A23V2250/61
Inventor 王淑敏步洪石王欢
Owner CHANGCHUN UNIV OF CHINESE MEDICINE
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