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Combination of mink viral enteritis inactivated vaccine and canine distemper live vaccine

A technology for viral enteritis and inactivated vaccines, applied in antiviral agents, active ingredients of heterocyclic compounds, antibody medical ingredients, etc., can solve problems such as animal stress, canine distemper antigen failure, and the inability to realize joint use

Active Publication Date: 2015-01-07
QILU ANIMAL HEALTH PROD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since each immunization in fur animal farming causes greater stress to the animals, it is extremely important to find a way to combine the two vaccines
Since the traditional mink viral enteritis inactivated vaccine uses formaldehyde solution inactivator, if it is used together with live canine distemper vaccine, it will cause the invalidation of canine distemper antigen, and the joint use cannot be realized

Method used

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  • Combination of mink viral enteritis inactivated vaccine and canine distemper live vaccine
  • Combination of mink viral enteritis inactivated vaccine and canine distemper live vaccine
  • Combination of mink viral enteritis inactivated vaccine and canine distemper live vaccine

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0050] (1) Preparation of seed poison for production

[0051] Canine distemper virus strain CDV-11 was inoculated on the Vero cell monolayer on the spinner bottle, and the amount of inoculation was 2% of the maintenance liquid volume. When the cytopathy reached 80%, it could be harvested, and passed on for another generation to prepare production seeds. poison.

[0052] (2) Propagation of virus liquid

[0053] The seed virus for production was used to inoculate the Vero cell monolayer on the spinner bottle, and the inoculation amount was 2% of the cell maintenance solution. Then the spinner bottle cells were cultured at 37°C with a rotation speed of 8-10r / h.

[0054] After inoculation, observe the lesions of the cells every day. When the lesions of the cells reach about 80%, they can be harvested, frozen and thawed once, and stored at -15°C. This is a semi-finished product.

[0055] (3) freeze-dried

[0056] After passing the qualified inspection of the semi-finished produ...

Embodiment 1

[0082] ——Preparation of poison seeds for production

[0083] 1. Propagation of virus seeds Take well-grown F81 cells or CRFK cells to pass passage according to conventional methods. After the cells grow into a single layer of 80%, discard the growth medium and replace it with a maintenance solution containing 1% virus seeds (containing 2% at 56 MEM of newborn bovine serum inactivated at ℃ for 30 minutes), placed at 37℃ to continue culturing, and harvested when about 80% of the cells showed typical stretch-net-like lesions, freeze-thawed once, quantitatively aliquoted, cryopreserved, and indicate the harvest Date, generation of virus species, etc.

[0084] 2. Identification of poisonous seeds The poisonous seeds that meet the above standards shall be used as the poisonous seeds for production.

[0085] 3. The subculture of poisonous seeds does not exceed 3 generations.

[0086] 4. Virus seeds are stored below -15°C, and the validity period is 24 months.

Embodiment 2

[0088] ——Manufacture of mink viral enteritis inactivated vaccine

[0089] 1. Selection of materials for making seedlings

[0090]F81 cells or CRFK cells that grow well and meet the current "Chinese Veterinary Pharmacopoeia" production and inspection cell standards are selected as materials for seedling production, and the cell generations are F6-F35 generations.

[0091] 2. Breeding of seedlings with venom

[0092] (1) Cleaning and sterilization of bioreactor and balance of microcarriers Clean the bioreactor and sterilize at 121° C. for 30 minutes. After the sterilization, put the sterilized microcarriers into the cell culture tank, put the balance medium MEM into the tank to the minimum culture volume, and balance for 24 hours. Calibration of the dissolved oxygen electrode 100% point is done during equilibration of the carrier.

[0093] (2) Cell inoculation and digestion and transfer Select vigorously growing spinner bottle cells, digest them with EDTA-trypsin dispersion, ...

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Abstract

The invention relates to a combination of a mink viral enteritis inactivated vaccine and a canine distemper live vaccine. The combination is the mink viral enteritis inactivated vaccine of using an alkylating agent as a virus inactivating agent, and is used as a diluent of the canine distemper live vaccine in the combination; and the two vaccines in the combination have a specific mixture ratio. Compared with traditional application methods of the two vaccines, the vaccine combination reaches the effects of dual prevention (preventing mink viral enteritis and canine distemper) in one injection, thus the vaccine cost is reduced, the animal stress reaction is reduced, the immune effect is improved, and the labor intensity of a feeder is relieved, thus obtaining significant economic benefits.

Description

technical field [0001] The invention relates to a combination of mink viral enteritis inactivated vaccine and canine distemper live vaccine. It belongs to the field of veterinary biological products. Background technique [0002] Mink viral enteritis is an acute, severe and highly contagious infectious disease caused by mink enteritis virus (Mink Enteritis Virus, MEV). It is mainly characterized by severe diarrhea. It was first discovered by Canadian scholar Sehofield in 1949 (Schofield F W. Virus enteritis in mink. Am Vet, 1949, 30:6512654.). In 1981, Jiang Tingxiu et al. (Jiang Tingxiu, Park Houkun, Wang Xilong, etc. Suspected the initial report of mink viral enteritis. Fur Animal Breeding, 1981, (2): 224.) first reported the occurrence of mink viral enteritis in my country, and the disease gradually spread throughout the country , bring huge economic losses to my country's mink industry (Gao Yun, Song Chunlin, Wu Yulin, etc. Mink Viral Enteritis (MEV) Epidemiological Inv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/23A61P31/20A61P31/14A61K39/175A61K31/40
Inventor 禚宝山宋晓飞李营秦绪伟田真王景伟王蕾鲍海忠
Owner QILU ANIMAL HEALTH PROD
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