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A kind of myrtle tissue culture rapid propagation technique

A technique of tissue culture, rapid propagation and myrtle, applied in the field of plant tissue culture, can solve problems such as no reports, and achieve the effect of solving the problem of supplying high-quality myrtle seedlings

Inactive Publication Date: 2016-03-09
李杨 +4
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the complete myrtle tissue culture rapid propagation technology has not been reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Collection and processing of explants: Select the full-bodied branches of the year's visceral buds from an excellent single plant of myrtle, soak in washing powder water for 15 minutes after removing leaves, rinse with tap water for 1 hour after brushing, and cut into 1-3 cm long strips The stems of axillary buds were first sterilized with 75% ethanol for 8 seconds, then washed 3 times with sterile water, then sterilized with 0.1% mercuric chloride solution for 20 minutes, washed 5 times with sterile water, and then set aside.

[0018] (2) Primary culture: Inoculate the above-mentioned stem segments on the primary culture medium, culture in total darkness at 28°C for 40 days, and then place under light for 10 hours a day with a light intensity of 2000lx until adventitious buds are induced. The primary medium is: MS+6-BA1.2mg / L+NAA0.4mg / L+CPPU0.05mg / L+Ad1.0mg / L+sucrose 25g / L+agar 4g / L, with a pH of 5.7.

[0019] (3) Proliferation culture: Transfer the above-mentioned...

Embodiment 2

[0023] (1) Collection and processing of explants: Select the full-bodied branches of the year's visceral buds from an excellent single plant of myrtle, soak in washing powder water for 30 minutes after removing leaves, rinse with tap water for 2 hours after brushing, and cut into 1-3 cm long strips The stems of axillary buds were first sterilized with 75% ethanol for 20 seconds, then washed 5 times with sterile water, then sterilized with 0.1% mercuric chloride solution for 15 minutes, rinsed 6 times with sterile water, and then set aside.

[0024] (2) Primary culture: Inoculate the above-mentioned stem segments on the primary culture medium, culture them in total darkness at 25°C for 30 days, and then place them under light for 11 hours a day with a light intensity of 2000 lx until adventitious buds are induced. The primary medium is: MS+6-BA1.0mg / L+NAA0.2mg / L+CPPU0.1mg / L+Ad2.0mg / L+sucrose 25g / L+agar 4g / L, with a pH of 5.8.

[0025] (3) Proliferation culture: Transfer the abo...

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Abstract

The invention discloses a rapid propagation technology for tissue culture of rhodomyrtus tomentosa (ait.) hassk and relates to a seedling culture method for obtaining stable seedlings of the rhodomyrtus tomentosa (ait.) hassk through a rapid asexual propagation technology. The rapid propagation technology comprises the following steps: acquiring and pre-treating an explant; performing primary culture; performing multiplication culture; performing rooting culture; hardening seedlings and transplanting. According to the rapid propagation technology, the seedlings of the rhodomyrtus tomentosa (ait.) hassk can be quickly propagated, the problem of the demand of large-scale growth of the wild native plant on the seedlings is solved, and the rapid propagation technology has a practical significance on full development of the wild plant with very high ornamental and edible values.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a myrtle tissue culture rapid propagation technology. Background technique [0002] Myrtle ( Rhodomyrtus tomentosa (Ait.) Hassk), also known as Renzi and Shanmin, is an evergreen and multi-flowered shrub of Myrtaceae Myrtaceae. It is a new food resource that has not yet been developed and utilized on a large scale. It has high research value and Application prospect. Our country is rich in wild myrtle plant resources. It is an excellent variety for greening barren hills and maintaining water and soil in South China. It is resistant to drought and barren and has strong adaptability. [0003] The utilization value of myrtle is high, and its fruit has attracted people's attention very early, but its development and utilization progress is slow. At present, some places in South China are developing large-scale myrtle planting, and high-quality myrt...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李杨王育荣陈培党黄炳成梁彩英
Owner 李杨
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