Rapid propagation method for Aletris stelliflora Hand.-Mzt
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A technology for pollen and cabbage, which is applied in the field of plant tissue culture, can solve the problems of no artificial large-scale planting, low seed setting rate, difficult germination and the like, and achieves the effects of small occupied space for reproduction, low cost and short cycle.
Inactive Publication Date: 2013-10-16
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI +1
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Problems solved by technology
Survive the winter with underground stems, sprout new shoots in spring, and flower stems are pumped from May to July, but the seed setting rate is low and it is not easy to germinate
Due to the limitation of seedlings, there is no relevant report on artificial large-scale planting
Method used
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Embodiment 1
[0019] A kind of star pollen fast breeding method, its steps are:
[0020] 1. Take the healthy and plump seeds of star pollen noodles, soak them in 1% mass ratio washing powder water for 4 minutes or 5 minutes or 6 minutes, then rinse them with tap water, and use 75% (volume ratio) alcohol on the ultra-clean workbench Soak the surface of the solution for about 10s or 15s or 20s or 25s or 30s, rinse with sterile water 3 times or 4 times or 5 times, and then disinfect with 0.1% (weight ratio) mercury chloride solution for 10min or 11min or 12min or 13min or 14min or 15min , washed with sterile water 3 times or 4 times or 5 times, and inoculated into MS medium.
[0021] 2. Illumination culture, the temperature is 25°C or 26°C or 27°C or 28°C, the light intensity is 1500Lx or 2000Lx or 2500Lx or 3000Lx, the light is 10h or 12h or 14h or 16h every day, cultivate for 30d or 40d or 50d or 60d, and the seeds germinate Seedlings with a height of 0.2cm or 0.3cm or 0.4cm or 0.5cm;
[0...
Embodiment 2
[0027] A kind of star pollen fast breeding method, its steps are:
[0028] 1. Take the healthy and plump seeds of star pollen noodles, soak them in 1% mass ratio washing powder water for 5 minutes, then rinse them with tap water, and soak them in 75% (volume ratio) alcohol solution on the ultra-clean workbench for 30 seconds , washed 5 times with sterile water, then sterilized with 0.1% (by weight) mercuric chloride solution for 15 minutes, washed 5 times with sterile water, and inoculated into MS medium.
[0029] 2. Illumination culture, the temperature is 25°C, the light intensity is 2000Lx, the light is 14 hours a day, and the culture is 50 days, the seedlings germinate with a height of 0.3cm;
[0030] 3. Under sterile conditions, cut the hypocotyls with a sterilized scalpel and inoculate them in the prepared cluster bud induction medium. The formula of the induction medium is: MS+6-BA2.0mg / L+NAA0. 4mg / L+agar 5000mg / L+sugar 30000mg / L;
[0031] 4. Light culture, the temper...
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Abstract
The invention discloses a rapid propagation method for Aletris stelliflora Hand.-Mzt. The method comprises the steps as follows: A, selecting seeds of the Aletris stelliflora Hand.-Mzt as an explant; B, disinfecting the explant with ethyl alcohol and mercury bichloride and washing the explant with sterile water; C, inoculating the explant to an MS medium for germination; D, cutting a lower germinated plumular axis as an explant for induction of a clustered bud under the aseptic condition; E, cutting the clustered bud into a plurality of small clustered buds and conducting multiplication culture; G, transferring a robust seedling to a root medium in the process of subculture, opening a bottle to harden the seedling after the root is grown, cleaning and then transplanting the seedling to a medium, preserving heat and moisture, and transplanting after the tissue cultured seedling survives. The obtained Aletris stelliflora Hand.-Mzt is good in genetic stability, occupies small propagation space, and is rapid in propagation, and ten thousand of Aletris stelliflora Hand.-Mzt can be propagated from one seed in a year. The problem of supply of the excellent seedling of Aletris stelliflora Hand.-Mzt is effectively solved in the planting of the Aletris stelliflora Hand.-Mzt. The built production system is stable, has a relatively short period, and can meet the requirement of the seedling in the large-scale planting.
Description
technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a rapid propagation method of star pollen sticks, which is a rapid propagation method of star pollen sticks by using a basic medium and adding different ingredients. Background technique [0002] Aletris stelliflora Hand.-Mzt (Aletris stelliflora Hand.-Mzt) Liliaceae Aletris stelliflora is a herbaceous plant, also known as oxtail, because its tender stems are thick with index fingers and about a foot long, resembling "oxtails". vegetable. Its tender stems are rich in vitamins, proteins, potassium, iron, calcium, phosphorus and other trace elements and dietary fiber. Its root or whole plant can also be used for medicine, which has the effects of clearing away heat and dampness, moistening the lungs and promoting blood circulation. According to current records, star pollen noodles are only found in alpine meadows, alpine swamps, forests, hillside shru...
Claims
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Application Information
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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 郭凤领吴金平丁自立邓晓辉陈磊夫邱正明
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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