The Rapid Propagation Method of Alpine Chives ovale

A technology of Chinese chives and high mountains, which is applied in the field of rapid propagation of Chinese chives to achieve the effects of saving time, short cycle and good genetic stability

Inactive Publication Date: 2016-06-15
INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to excavate a large amount of existing wild resources of Chives ovalifolia, and under the natural state with the characteristic that asexual reproduction is main, utilize tissue culture technique, provide the method for rapid propagation of Alpine Chinese chives ovale seedlings, adopt this method to be able to Effectively solve the seedling problem of Chinese chives and provide guarantee for the artificial large-scale planting of Chinese chives

Method used

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  • The Rapid Propagation Method of Alpine Chives ovale
  • The Rapid Propagation Method of Alpine Chives ovale
  • The Rapid Propagation Method of Alpine Chives ovale

Examples

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Embodiment 1

[0025] The rapid propagation method of alpine Chinese chives, the steps of which are:

[0026] 1. After the seedlings of the Chinese chives ovale are eliminated in the first ten days of April and before the scape is formed, the healthy and full bulb discs are taken, and the taupe to dark brown reticulated bulb skin and roots are peeled off, and washed with 1% mass ratio washing powder water Soak for 4min or 5min or 6min, rinse with tap water; peel off the outermost layer of bulbs, soak in 1% mass ratio washing powder water for 4min, 5min or 6min, rinse with tap water; use 75% volume on the ultra-clean workbench Soak in specific alcohol solution for 10s or 15s or 20s or 25s or 30s, sterilize with 0.1% by weight mercuric chloride solution for 3min or 4min or 5min, rinse with sterile water 3 times or 4 times or 5 times;

[0027] 2. Blot the moisture on the surface of the explants with sterile paper, use a sterilized scalpel to peel off the bulb disc of Chive ovata to a diameter o...

Embodiment 2

[0032] The rapid propagation method of alpine Chinese chives, the steps of which are:

[0033]1. After the seedlings of the Chinese chives ovale are eliminated in the first ten days of April and before the scape is formed, the healthy and full bulb discs are taken, and the taupe to dark brown reticulated bulb skin and roots are peeled off, and washed with 1% mass ratio washing powder water Soak for 5 minutes, rinse with tap water; peel off the outermost layer of bulbs, soak with 1% mass ratio washing powder water for 5 minutes, and rinse with tap water; soak with 75% by volume alcohol solution for 30 seconds on an ultra-clean workbench, use Disinfect with 0.1% by weight mercuric chloride solution for 4 minutes, rinse with sterile water 5 times;

[0034] 2. Blot the moisture on the surface of the explants with sterile paper, use a sterilized scalpel to peel off the bulb disc of Chive ovata to a diameter of 0.3 cm or 0.4 cm or 0.5 cm, and insert it vertically in the prepared ind...

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Abstract

The invention discloses a rapid propagation method of Allium ovalifolium on high mountain. The method comprises the following steps: A. selecting the plateau of Allium ovalifolium on high mountain as an explant; B. peeling the plateau until reaching the diameter of 0.3-0.5cm with a sterilized scalpel, inserting into an induction medium in a polarity direction; C. cultivating at constant temperature under illumination until a clump shoot grows; D. cutting the clump shoot into a plurality of small clump shoots and transferring into a proliferation medium for multiplication of subculture; E. transferring a strong seedling into a root medium in a subculture cultivation process, opening a bottle for hardening seedling after rooting, cleaning, transferring into matrix, keeping the temperature and the humidity, and transplanting after the tissue culture seedling is survival. The acquired Allium ovalifolium has good hereditary stability, small occupied area for propagation and high propagation speed, one plateau per year can reproduce millions of Allium ovalifolium seedlings, the supplying problem of high-quality Allium ovalifolium seedlings in Allium ovalifolium planting can be effectively solved, the built production system is stable, the period is short, and the scale seedling planting requirement can be met.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture, and more specifically relates to a method for rapid propagation of Allium ovalifolia by adding different components to a basic culture medium by using the bulb disc of Alpine allium ovalifolia as an explant. Background technique [0002] Allium ovalifolium Hand.-Mzt., also known as deer ear leek, is commonly known as "Sky Garlic" in Wufeng, Changyang, Hubei and other places, and "Shanpian" in Hanyuan, Sichuan. It is a perennial herb. Distributed in Hubei, Sichuan, Hunan and other places in China, it grows in areas with an altitude of 1800-2100m, and mostly grows in damp hillsides and ditch edge forests. Oval leaf leek has high nutritional value, tender and delicious vegetable quality, spicy taste, and because the growth environment is original ecology, it is an organic vegetable, and local people eat young leaves. The whole herb of Chive ovata is a traditional Chinese medicine with ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 郭凤领吴金平邓晓辉郭世喜陈磊夫黄毅华邱正明
Owner INST OF ECONOMIC CROP HUBEI ACADEMY OF AGRI SCI
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