Kit for detecting foot-and-mouth disease virus type O and preparation method thereof

A detection kit and foot-and-mouth disease technology, which is applied in the detection kit and preparation field of foot-and-mouth disease type O virus, can solve the problems of low sensitivity, inability to type, and long time consumption

Active Publication Date: 2014-12-24
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
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  • Claims
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Problems solved by technology

[0009] The invention provides a detection kit for detecting foot-and-mouth disease type O virus which can overcome the deficiencies of the prior art such as inability to type, low sensitivity and long time consumption

Method used

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  • Kit for detecting foot-and-mouth disease virus type O and preparation method thereof
  • Kit for detecting foot-and-mouth disease virus type O and preparation method thereof
  • Kit for detecting foot-and-mouth disease virus type O and preparation method thereof

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Embodiment Construction

[0021] The present invention is explained in detail below in conjunction with embodiment.

[0022] 1. Preparation of Sequences

[0023] According to the GeXP primer design requirements and the FMDV-O genome published by NCBI, select the conserved region to design specific primers, and form specific chimeric primers by adding universal primers, while the universal primer sequences belong to non-biologically derived nucleotide sequences , in addition to synthesizing the universal primer sequence, and adding a Cy5 fluorescent label to the 5' end of the upstream universal primer. The primer sequences capable of specifically amplifying foot-and-mouth disease type O virus nucleic acid of the present invention are: AGGTGACACTATAGAATAGTGACTGAACTGAACTGAACTGCTTTACCGCAT, which is named FMDV-O-F in the present invention; GTACGACTCACTATAGGGAGACATGTCCTCCTGCATCTG, which is named FMDV-O-R in the present invention. Universal primers used in the present invention: Cy5 AGGTGACACTATAGAATA, named...

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Abstract

The invention discloses primers for detecting a foot-and-mouth disease virus type O, a detection kit prepared by using the primers and a preparation method of the kit. The gene sequences of the primers for detecting the foot-and-mouth disease virus type O are SEQ ID No.1, SEQ ID No.2, SEQ ID No.3 and SEQ ID No.4. Related experiments indicate that nucleic acid of the foot-and-mouth disease virus type O can be specifically quickly amplified by adopting the primer sequences; a specific band is detected by nucleic acid electrophoresis and has specificity. The detection kit can be used for performing high-flux qualitative, determinatus and quantitative detection on foot-and-mouth disease virus. Compared with other existing methods and kits, the method and kit provided by the invention have great advantages in port quarantine, animal circulation quarantine and laboratory diagnosis.

Description

technical field [0001] The invention relates to a primer, a detection kit and a preparation method that can be used for detecting foot-and-mouth disease type O virus. Background technique [0002] Foot-and-mouth disease (FMD) is an acute and severe infectious disease of animals caused by foot-and-mouth disease virus (FMDV), which mainly harms pigs, cattle, and sheeppox cloven-hoofed animals, with a very high incidence , has caused huge political and economic losses, so it is listed as the first class A infectious disease by the World Health Organization (OIE). FMDV can be divided into 7 serotypes, namely A, O, C, Asia 1, SAT 1, SAT 2 and SAT 3, and each serotype has many different subtypes, and each serotype There is no cross-immunity between them, and there is only partial cross-immunity between subtypes of the same serotype. [0003] The typing and differential diagnosis of foot-and-mouth disease virus has always been a research hotspot. Because there is no cross-protec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/68C12N15/11C12R1/93
CPCC12Q1/701C12Q1/686C12Q1/70C12Q2531/113C12Q2565/125
Inventor 张强卢昌赵志荀吴国华颜新敏李应国岳华周晓黎李健朱海霞代雪玲田波芦晓立高顺平王曼
Owner LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
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