Primers and kit for detecting Asia1-type foot-and-mouth disease virus and preparation method of primers and kit

Abstract

The invention discloses primers and kit for detecting an Asia1-type foot-and-mouth disease virus (FMDV) and a preparation method of the primers and the kit. The kit for detecting the Asia1-type FMDV comprises four amplification primers used for a GeXP multiplex gene analysis system. Related experiments show that by sequences of FMDV-Asia1-F and FMDV-Asia1-R, the nucleic acid of the Asia1-type FMDV can be specifically and rapidly amplified, specific bands are detected by virtue of DNA electrophoresis and the specificity is extremely high. The Asia1-type FMDV can be subjected to high-throughput specific detection by virtue of the GeXP multiplex gene analysis system which has sensitivity of 10<2> copies.

Description

technical field [0001] The invention relates to a primer, a detection kit and a preparation method which can be used for detecting foot-and-mouth disease Asia 1 type virus. Background technique [0002] Foot-and-mouth disease (FMD) is an acute and severe infectious disease of animals caused by foot-and-mouth disease virus (FMDV), which mainly harms pigs, cattle, and sheeppox cloven-hoofed animals, with a very high incidence , has caused huge political and economic losses, so it is listed as the first class A infectious disease by the World Health Organization (OIE). FMDV can be divided into 7 serotypes, namely A, O, C, Asia 1, SAT 1, SAT 2 and SAT 3, and each serotype has many different subtypes, and each serotype There is no cross-immunity between them, and there is only partial cross-immunity between subtypes of the same serotype. [0003] The typing and differential diagnosis of foot-and-mouth disease virus has always been a research hotspot. Because there is no cross-...

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Problems solved by technology

[0009] The invention provides a kind of primers that can overcome the shortcomings of the prior art, such as inability to type, low sensitivity, and long time consumption, and can be used to detect foot-and-mouth disease Asia type 1 virus, a detection kit including these primers, and the kit is non-disease-resistant Use for diagnostic purposes

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