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Evaluation method of effect of penehyclidine hydrochloride on calcium ion in animal model of dysmenorrhea

A technology of penehyclidine hydrochloride and animal models, which is applied in the direction of biochemical equipment and methods, and the measurement/testing of microorganisms, and can solve problems such as the mechanism of dysmenorrhea treatment that has not been fully understood

Active Publication Date: 2016-03-30
SICHUAN PROVINCIAL PEOPLES HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] According to experimental research, penehyclidine hydrochloride may have a certain effect on the treatment of primary dysmenorrhea, but its mechanism for the treatment of dysmenorrhea is not completely clear, and a new evaluation method is needed to verify the effect of penehyclidine hydrochloride on the treatment of dysmenorrhea. Mechanisms of treating dysmenorrhea

Method used

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  • Evaluation method of effect of penehyclidine hydrochloride on calcium ion in animal model of dysmenorrhea
  • Evaluation method of effect of penehyclidine hydrochloride on calcium ion in animal model of dysmenorrhea

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Take SD rat uterine cells and prepare a cell suspension with a concentration of 100,000 cells / ml; add the calcium ion fluorescent probe reagent Fura2-AM to the cell suspension to a final concentration of 5umol / L, shake it away from light, and load for 20 minutes ; Obtain a blank cell suspension.

[0024] Take a blank cell suspension, add 20ul of oxytocin containing 0.001 unit, and mix for 5 min to obtain an animal model cell suspension. The animal model cell suspension was divided into three groups. One group is the positive control group with 2×10 control reagents -5 mol / L of atropine, the final concentration of atropine is 0.8mg / ml to act for 10 minutes, one group is the experimental group with a concentration of 10 -7 The final concentration of mol / L penehyclidine hydrochloride was 0.0067mg / ml for 10 minutes, and one group was the model group without adding drugs.

[0025] Take blank cell suspension, positive control group, experimental group and model group, and measure...

Embodiment 2

[0027] Take SD rat uterine cells to prepare a cell suspension with a concentration of 150,000 cells / ml; add the calcium ion fluorescent probe reagent Fura2-AM to the cell suspension to a final concentration of 5umol / L, shake in the dark, and load for 30 minutes ; Obtain a blank cell suspension.

[0028] Take a blank cell suspension, add 20ul of 0.001 unit of oxytocin, and mix for 5 minutes to obtain an animal model cell suspension. The animal model cell suspension was divided into three groups. One group is the positive control group with control reagents, 2×10 -5 The mol / L atropine acts for 15 minutes, and the final concentration of atropine is 0.8mg / ml. One group adds 5×10 to the experimental group -7 The mol / L penehyclidine hydrochloride was treated for 15 minutes, the final concentration of penehyclidine hydrochloride was 0.013mg / ml, and one group was the model group without adding drugs.

[0029] Take blank cell suspension, positive control group, experimental group and mode...

Embodiment 3

[0031] Take SD rat uterine cells and prepare a cell suspension with a concentration of 200,000 cells / ml; add the calcium ion fluorescent probe reagent Fura2-AM to the cell suspension to a final concentration of 5umol / L, shake in the dark, and load for 40 minutes ; Obtain a blank cell suspension.

[0032] Take a blank cell suspension, add 20ul of oxytocin containing 0.001 unit, and mix for 5 min to obtain an animal model cell suspension. The animal model cell suspension was divided into three groups. One group is the positive control group with control reagents, 2×10 -5 The mol / L atropine acts for 20 minutes, and the final concentration of atropine is 0.8mg / ml. One group adds 20×10 to the experimental group -7 The mol / L penehyclidine hydrochloride acts for 20 minutes, and the final concentration of penehyclidine hydrochloride is 0.02mg / ml. One group is the model group without adding drugs.

[0033] Take blank cell suspension, positive control group, experimental group and model g...

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Abstract

The invention discloses an evaluation method of influences of penehyclidine hydrochloride to calcium ions in dysmenorrhea animal model cells. The method comprises the following steps: obtaining a blank cell suspension; adding oxytocin into the blank cell suspension, and mixing for acting for 5 to 15 minutes so as to obtain an animal model cell suspension; dividing the animal model cell suspension into three groups, and adding reagents in equal volume into three groups of animal model cell suspension, wherein one group is a positive control group in which a contrast reagent is added and acts for 10 to 20 minutes, one group is an experimental group in which penehyclidine hydrochloride in different doses is added and acts for the same time with the positive control group, and one group is a model group without adding the reagent; and contrasting differences of the concentration of the calcium ions in the cells of the experimental group with the concentration of the calcium ions in the cells of the model group and the positive control group. The embodiment of the invention can reveal the influences of penehyclidine hydrochloride to the concentration of the calcium ions in the dysmenorrhea animal model cells, and can ensure that the efficacy of penehyclidine hydrochloride in different concentrations is evaluated by contrasting the concentration of the calcium ions in the cells of the experimental group with the concentration of the calcium ions in the cells of the positive control group and the model group.

Description

Technical field [0001] The invention relates to a method for evaluating the effect of penehyclidine hydrochloride on calcium ions in dysmenorrhea animal model cells. Background technique [0002] Dysmenorrhea is the periodic lower abdominal pain that occurs before and after the menstrual period. It often occurs before and during the menstrual period, and occasionally occurs within a few days after the menstrual period. The lower abdominal pain is cramping pain and fullness, which can radiate to the lumbosacral area, inner thigh and around the anus. It may be accompanied by pale complexion, nausea, vomiting, chills in the whole body or lower abdomen, frequent stools, and collapse may occur in severe pain. [0003] Dysmenorrhea is one of the most common symptoms in gynecology. The common manifestation is cramping pain in the lower abdomen and general malaise before and after menstruation or during menstruation. Due to the high incidence, especially in female adolescence, it is abou...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/02
Inventor 廖治肖洪涛张远张丽娟
Owner SICHUAN PROVINCIAL PEOPLES HOSPITAL
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