Applications of artificially synthesized gene-derived recombinant protein rLj-RGD4 in anti-tumor drugs

A technology for artificial synthesis and recombination of proteins, used in antitumor drugs, medical preparations containing active ingredients, applications, etc.

Inactive Publication Date: 2014-12-24
LIAONING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

rLj-RGD4 is a brand-new artificially synthesized gene recombinant protein. There is no research and report at home and abroad. This invention is the first discovery

Method used

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  • Applications of artificially synthesized gene-derived recombinant protein rLj-RGD4 in anti-tumor drugs
  • Applications of artificially synthesized gene-derived recombinant protein rLj-RGD4 in anti-tumor drugs
  • Applications of artificially synthesized gene-derived recombinant protein rLj-RGD4 in anti-tumor drugs

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Experimental program
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Embodiment Construction

[0021] 1, lj-rgd4 The gene was connected with the pET23b vector, and the positive transformants were screened and identified after being transformed into Escherichia coli.

[0022] In order to produce a fusion protein with a histidine tag, pET-23b was selected as the vector for gene cloning. The specific operation of cloning is as follows:

[0023] (1) Recovery and sequencing of target genes: TaKaRa PCR Fragment Recovery Kit was used to recover target genes; the recovered DNA was sequenced, and this work was completed by Dalian Bao Biological Engineering Co., Ltd.

[0024] (2) Plasmid extraction: Use the plasmid extraction kit of Baobio.

[0025] (3) Ligation of the DNA fragment of the target gene to the vector pET23b: Since the designed primers contain Nde I and Hin d III restriction sites, and these two restriction sites are also multiple cloning sites of pET23b, which makes it possible to connect the DNA fragment of the target gene with the vector pET23b.

[0026] (4) ...

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Abstract

The invention discloses'applications of an artificially synthesized gene-derived recombinant protein rLj-RGD4 in anti-tumor drugs', belonging to the field of biotechnology and relating to gene cloning and protein expression of an artificially synthesized gene lj-rgd4, dose-dependent potent angiogenesis inhibition and tumor inhibition functions of the recombinant protein rLj-RGD4, and applications of the recombinant protein in the antitumor drugs as an angiogenesis inhibitor.

Description

technical field [0001] "Application of recombinant protein rLj-RGD4 derived from artificially synthesized genes in anti-tumor drugs" belongs to the field of biotechnology, involving the gene cloning and protein expression of recombinant RGD motif protein Lj-RGD4, and rLj-RGD4 inhibiting angiogenesis The signal transduction pathway of integrin highly expressed on the surface of endothelial cells can strongly inhibit angiogenesis and its application as an angiogenesis inhibitor in anti-tumor pharmaceuticals. Background technique [0002] Angiogenesis is fundamental to tumor growth and metastasis. When the blood vessel volume reaches 1-2 mm 3 When , the tumor exhibits an angiogenic phenotype and replenishes blood vessels from the surrounding stroma. The growth and development of primary solid tumors are highly dependent on angiogenesis. In preclinical models, anti-angiogenic drugs targeting tumor vasculature have been shown to hinder or delay tumor growth, and even promote t...

Claims

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Application Information

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IPC IPC(8): C12N15/12C07K14/46A61K38/17A61P35/00A61P9/00
Inventor 李庆伟王继红吕莉郑媛媛张安伟管萧玉杨兰邵钫钰白娟
Owner LIAONING NORMAL UNIVERSITY
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