Chitosan-grafted polylactic acid composite microspheres loaded with hydrophilic and hydrophobic biomolecules and preparation method thereof

Abstract

The invention discloses a chitosan grafted polylactic acid composite microsphere simultaneously carried with hydrophilic and hydrophobic biological molecules and a preparation method of the chitosan grafted polylactic acid composite microsphere, and belongs to the technical field of biological medical materials, functional macromolecular microspheres and medicament control release. The composite microsphere consists of chitosan grafted polylactic acid, hydrophilic biological molecules and hydrophobic biological molecules. The preparation method of the composite microsphere comprises the steps of dissolving the hydrophobic biological molecules into a chitosan grafted polylactic acid solution to obtain a core-shell type micellar solution, then adding the hydrophilic biological molecules, dropping the micellar solution into an oil phase under the action of a surface active agent to obtain water-covering oil type mixed emulsion, adding a crosslinking agent to further crosslink the micellar solution to obtain a sphere, and forming the chitosan grafted polylactic acid composite microsphere simultaneously carried with hydrophilic and hydrophobic biological molecules. The preparation method disclosed by the invention obtains the novel composite microsphere, so that partitioning distribution of the hydrophilic and hydrophobic biological molecules is realized, and the chitosan grafted polylactic acid composite microsphere can be expected to be widely applied in the field of medicament control release and tissue defect repairing.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, functional polymer microspheres and drug controlled release, in particular to chitosan-grafted polylactic acid composite microspheres carrying hydrophilic and hydrophobic biomolecules and a preparation method thereof. Background technique [0002] The growth and development of human tissues and organs are affected by the synergistic effects of various biomolecules. In order to use tissue engineering strategies to repair diseased tissues, it is usually necessary to co-deliver multiple biomolecules to the defect site. With the continuous emergence of new material structure design methods, people have developed a series of co-delivery carriers for biomolecules (Zhou Ping, Wang Changchun, Zhong Lingling, etc. Targeted composite nano-drug preparations that simultaneously carry hydrophilic and hydrophobic drugs and their Preparation method. CN 102397256A, 2012), its size ranges from tens ...

AI Technical Summary

Problems solved by technology

However, the preparation of graft copolymers of chitosan and polylactic acid by chemical modification is by no means easy, and the reasons include: first, chitosan has a high glass transition temperature and decomposes before it starts to melt, This makes it very difficult to copolymerize it with polylactic acid using traditional melt polymerization techniques; The solvent of similar materials, which makes the copolymerization of the two by the solution polymerization process often fails due to the heterogeneous reaction

Therefore, although chitosan and polylactic acid composites exhibit better performance than single materials, they are still not suitable for the co-delivery of hydrophilic and hydrophobic biomolecules.

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