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Method for improving tensile strength of collagen fiber material

A collagen fiber and tensile strength technology, applied in the field of medical biomaterials, can solve the problems of complex equipment, inability to realize large-scale sample preparation, and high operating conditions, and achieve high tensile strength

Active Publication Date: 2014-12-17
GUANGZHOU TRAUER BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this preparation method induced by electric field, magnetic field or hydrodynamics also has shortcomings, such as high requirements for operating conditions, complex equipment required, and inability to achieve large-scale sample preparation, etc.

Method used

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  • Method for improving tensile strength of collagen fiber material
  • Method for improving tensile strength of collagen fiber material
  • Method for improving tensile strength of collagen fiber material

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Under the condition of ice bath, 0.5 mol / L acetic acid aqueous solution was used to dissolve the type Ⅰ collagen extracted from grass carp skin. The pH of the collagen solution was adjusted to 7.0 by dialysis against the phosphate buffer solution at 4° C. to obtain a collagen solution with a concentration of 3 mg / mL. The collagen solution was transferred to a 10mL centrifuge tube, and centrifuged at 25°C and a relative centrifugal force of 3g for 5 minutes. Then the centrifuge tube was carefully and vertically taken out and vertically transferred to an incubator, and left to stand at 30° C. for 24 hours. Take out the collagen gel from the centrifuge tube, transfer it to 1% glutaraldehyde solution for cross-linking for 24 hours, then take out the collagen gel and wash it repeatedly with distilled water; then, at room temperature, fix one end of the collagen gel vertically, and continuously apply it to the other end Vertical downward pulling force of 0.7 N, after natural...

Embodiment 2

[0035] Under the condition of ice bath, 0.5 mol / L acetic acid aqueous solution was used to dissolve the type Ⅰ collagen extracted from grass carp skin. The pH of the collagen solution was adjusted to 7.0 by dialysis against the phosphate buffer solution at 4° C. to obtain a collagen acid solution with a concentration of 3 mg / mL. Transfer the collagen solution to a 10mL centrifuge tube and centrifuge at 25°C for 5 minutes at a centrifugal force of 3g. Then the centrifuge tube was carefully and vertically taken out and vertically transferred to an incubator, and left to stand at 30° C. for 24 hours. Take out the collagen gel from the centrifuge tube, transfer it to 1% glutaraldehyde solution for cross-linking for 24 hours, then take out the collagen gel and wash it repeatedly with distilled water; then transfer the collagen gel to a blast drying oven after natural drying at room temperature for 24 hours The collagen fiber material was obtained after drying at a temperature of 3...

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Abstract

The invention discloses a method for improving the tensile strength of a collagen fiber material. The method comprises the following steps: 1) dissolving natural collagen with acetic acid solution and dialyzing a phosphate buffer solution to obtain a collagen solution with collagen concentration of 1-20mg / mL and pH value of 6.0-9.0; 2) transferring the collagen solution into centrifugal tubes in a centrifuge with a horizontal rotor, and centrifuging for 5 to 120 minutes; 3) placing the centrifugal tubes vertically in a water bath or an incubator, standing for 2-48 h to get collagen gel; 4) placing the collagen gel in a 0.5%-10% aqueous solution of glutaraldehyde for cross-linking for 1-24 h, and then repeatedly rinsing with distilled water; and 5) naturally drying for 12 to 36 h, and drying in a blast drying box for 12-36 h to obtain the collagen fiber material. The collagen fiber prepared by the method of the invention has collagen fibers in consistent arrangement direction, and the tensile strength of the material can be improved significantly.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a method for improving the tensile strength of collagen fiber materials. Background technique [0002] In recent years, natural collagen (especially type I collagen) has been more and more widely used in the fields of biomedical materials, medical tissue engineering, and medical cosmetology. Such as the application of collagen sponge in wound repair and rapid hemostasis; the application of collagen glue in facial skin wrinkle removal, targeted drug preparation and drug sustained release; collagen composite materials in artificial blood vessels, artificial skin and bones, artificial corneal transplantation and Applications in medical tissue engineering such as artificial Achilles tendons and tendons / ligaments. [0003] Good tensile strength is an important indicator required for medical tissue engineering materials. For example, after artificial Achilles...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08L89/00C08K5/07D01F4/00
Inventor 汪海波赵燕汪海婴
Owner GUANGZHOU TRAUER BIOTECH
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