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High temperature-resistant Candidasp. FD-1 for phenolated water degradation

A technology of Candida, Candida, applied in the field of yeast

Inactive Publication Date: 2014-12-17
范明星
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Scholars at home and abroad have done a lot of research on how to use microorganisms to remove phenol and its derivatives in wastewater, and have obtained some strains that can degrade phenol solutions, but basically they are rarely used in the actual treatment of industrial phenol-containing wastewater.

Method used

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  • High temperature-resistant Candidasp. FD-1 for phenolated water degradation
  • High temperature-resistant Candidasp. FD-1 for phenolated water degradation
  • High temperature-resistant Candidasp. FD-1 for phenolated water degradation

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Embodiment Construction

[0021] Isolation, cultivation and identification of Candida sp.FD-1 strain

[0022] 1.1 Medium

[0023] 1.1.1 LB medium Peptone 1%, NaCl 1%, yeast extract 0.5%, agar (solid medium) 1.5%.

[0024] 1.1.2 Inorganic salt screening medium K 2 HPO 4 0.5g / L, NaCl 0.2g / L, MnSO 4 ·H 2 0 0.02g / L, CaCl 0.05g / L, KH 2 PO 4 0.5g / L, MgSO 4 ·7H 2 O 0.1g / L, Fe 2 (SO 4 ) 3 ·H 2 O 0.005g / L, (NH 4 ) 2 SO 4 1g / L, phenol 1g / L, agar 1.5% (solid medium).

[0025] 1.2 Isolation and enrichment of strains

[0026] Take the sludge directly from the industrial phenol-containing wastewater pool, and dilute it by 10 1 、10 2 、10 3 、10 4 、10 5 、10 6 After doubling, spread on LB plate for culture, pick a single colony and expand culture with LB liquid medium.

[0027] 1.3 Domestication of strains

[0028] When the bacteria in the LB liquid medium have reached the logarithmic growth phase after being cultivated, the bacterial liquid is gradually inserted into the LB l...

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Abstract

The invention discloses high temperature-resistant Candidasp. FD-1 for phenolated water degradation. The high temperature-resistant Candidasp. FD-1 is named as Candidasp. FD-1, is preserved in China center for type culture collection which is an international depository authority appointed by the national patent office and is located in Chinese Wuhan university in the Wuhan city, is preserved on May 4, 2014, and has an accession number of CCTCC NO: M2014180. The high temperature-resistant Candidasp. FD-1 for phenolated water degradation has the advantages that 1, the domesticated strain FD-1 has greatly improved phenol degradation efficiency and has the highest adaptable phenol complete-degradation concentration of 1000mg / L, and 2, the Candidasp. FD-1 has the optimal phenol-degradation culture temperature of 25-37 DEG C and the optimal pH of 6.0-7.0, has a wide suitable temperature range and a wide suitable pH range, can resist high-content salt, has the highest salt resistance of 5% considerably higher than the seawater average salinity (3.5%) and is microorganism having a good application prospect.

Description

technical field [0001] This invention relates to the field of yeast. Background technique [0002] Phenol is the main pollutant in industrial wastewater such as papermaking, coking, oil refining, plastics, textiles, ceramics, etc. Phenol and its derivatives are aromatic compounds, protoplasmic poisons, and have toxic effects on organisms [1] , and is difficult to be degraded. Phenolic compounds are listed by the US Environmental Protection Agency as one of the 129 priority pollutants blacklisted ] . Since the toxicity of phenols involves the growth and reproduction of aquatic organisms, it pollutes drinking water sources and causes serious pollution to water bodies [3] . Phenol-containing wastewater is listed as one of the key harmful wastewater in my country's water pollution control. Methods for removing phenolic substances in industrial wastewater include microbial degradation, extraction, activated carbon adsorption, and chemical oxidation, among which the use of mi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F101/34C12N1/16C12R1/72C02F3/34
Inventor 李小燕范明星
Owner 范明星
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