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Method for extracting polysaccharide from chlorella

A technology of chlorella and polysaccharide, applied in the chemical industry, can solve the problems of high cost, long time, and no practical application value.

Inactive Publication Date: 2014-12-10
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Liu Yongding, Shen Yinwu, etc. invented "a method for extracting polysaccharides from cyanobacteria with water bloom" (CN200410012927.3), which is to add 15 to 20 times the weight of distilled water to the cyanobacteria powder, fully moisten it, soak it for 2-3 hours, and then At 70-92°C, use a stirrer to continuously stir and extract for 3-5 hours, collect the supernatant, remove protein, and precipitate cyanobacterial polysaccharides with alcohol. There are many similar methods, such as CN200510038387.0, CN200810015880.4, etc. Polysaccharide extraction methods for other algae are also disclosed. On the one hand, these methods are not designed for Chlorella, and the extraction yield of polysaccharides in Chlorella is relatively poor, and has no practical application value; on the other hand, they all use high temperature Hot water extraction takes too long, although the use of protease as a catalyst can increase the extraction speed, but the cost is greatly increased

Method used

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Experimental program
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Effect test

Embodiment 1

[0022] The present invention extracts polysaccharides from Chlorella sp.-YL through the following steps:

[0023] A. Preparation of culture medium

[0024] Add 1.5g of sodium nitrate, 0.065g of dipotassium hydrogen phosphate, 0.055g of magnesium sulfate, 0.0072g of calcium chloride, 0.0066g of citric acid, 0.006g of ferric ammonium citrate, 0.0012g of disodium edetate, Sodium carbonate 0.02g, trace element solution 1.0ml.

[0025] Wherein the trace element solution adds boric acid 2.86g, manganese chloride tetrahydrate 1.86g, zinc sulfate heptahydrate 0.22g, sodium molybdate dihydrate 0.39g, copper sulfate pentahydrate 0.08g, cobalt nitrate hexahydrate 0.05g per liter of water, Stir until completely dissolved.

[0026] The medium solution used for the secondary culture was additionally added with 1% (mass fraction) of glucose on the basis of the above, that is, 10 g of glucose was added per liter of water.

[0027] B. Cultivation of Chlorella

[0028] (1) Primary culture: ...

Embodiment 2

[0040]The present invention extracts polysaccharides from Chlorella sp.-YL through the following steps:

[0041] A. Preparation of culture medium

[0042] Add 2.6g of sodium nitrate, 0.037g of dipotassium hydrogen phosphate, 0.015g of magnesium sulfate, 0.01g of calcium chloride, 0.0027g of citric acid, 0.002g of ferric ammonium citrate, 0.0038g of disodium edetate, Sodium carbonate 0.04g, trace element solution 1.8ml, wherein the trace element solution is added H 3 BO 3 2.86g, MnCl 2 4H 2 O 1.81g, ZnSO 4 ·7H 2 O 0.222g, Na 2 MoO 4 0.39g, CuSO 4 ·5H 2 O0.079g, Co(NO 3 ) 2 .6H 2 O 0.049g, stir well after completely dissolved.

[0043] B. Cultivation of Chlorella

[0044] (1) Primary culture: put the Chlorella species into a 200mL Erlenmeyer flask containing glucose-free culture solution for static culture, and shake the flask 3 times a day to ensure uniform light and CO 2 access and O 2 Timely release, the light intensity is controlled at 4500 lux, and the tempe...

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Abstract

The invention discloses a method for extracting polysaccharide from chlorella. The method comprises the following steps: performing multi-stage culture on chlorella, and extracting the cultured chlorella by using an inorganic alkali solution, wherein a culture medium without glucose is adopted in one-stage culture of chlorella. By adopting the method disclosed by the invention, the biomass yield of chlorella is high, and the main component, namely, polysaccharide, of chlorella can be easily, rapidly and conveniently obtained. Compared with a conventional method, the method is feasible, convenient to operate, safe and reliable, simple in equipment, low in cost and applicable to industrial on-scale production.

Description

technical field [0001] The invention relates to a method for extracting and obtaining chlorella polysaccharides from chlorella, belonging to the field of chemical industry. Background technique [0002] Chlorella belongs to Chlorophyta, Chlorophyceae, Chlorellaceae, Chlorellaceae, and Chlorella genus. It is a spherical single-celled freshwater algae with a diameter of 3 to 8 microns. It is an efficient photosynthetic plant. The distribution is extremely wide. Chlorella is rich in various nutrients, including 10% to 25% of carbohydrates (sugars), which have activities such as anti-oxidation, anti-virus, anti-tumor, and improving body immunity. Similar polysaccharide products currently on the market are extracted from large seaweeds, such as red algae and brown algae, and there are few successful precedents for the research on chlorella polysaccharides. [0003] The reason is mainly limited by two factors. On the one hand, the biomass of Chlorella is low during photoautotrop...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/04C08B37/00C12R1/89
Inventor 徐小琳贺莹莹代斌王长海
Owner SHIHEZI UNIVERSITY
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