Preparing method of corn gluten polypeptide for fodder
A technology of corn protein and production method, which is applied in the direction of vegetable protein processing and the like, can solve the problems of insoluble protein being difficult, wasteful, polluting the environment, etc., and achieve the effects of good palatability, outstanding efficacy and rich nutrition.
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[0010] specific implementation plan
[0011] The bacterial strain Aspergillus sojae CICC40357 used in the present invention was purchased from China Industrial Microorganism Culture Collection and Management Center.
[0012] Take 6B X The wort was sterilized at 121° C. for 20 minutes, cooled to 28° C., inoculated with Aspergillus sojae, and cultivated on a shaker at 160 rpm and 28° C. for 20 hours to obtain Aspergillus sojae seed liquid.
[0013] Expand corn gluten powder, weigh a certain amount of expanded corn gluten powder, add 15 times the mass of water, stir evenly, adjust the pH to 7.0, sterilize at 121°C for 20 minutes, cool down to 50°C, and add 80% per gram of raw material Add neutral protease in the proportion of the unit, hydrolyze at 50°C for 6 hours, then boil for 15 minutes, cool down to 28°C, inoculate the seed liquid of Aspergillus sojae with 10% inoculation amount, and ferment on a shaker at 160rpm and 28°C for 30 hours , and centrifuge to obtain a clear liq...
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