Detection kit for distinguishing three aquatic source aeromonas composite groups

Abstract

The invention discloses a detection kit for distinguishing three aquatic source aeromonas composite groups, and aims at providing a detection kit which is capable of simultaneously screening three common aquatic source aeromonas composite groups, namely an aeromonas hydrophila composite group, an aeromonas sobria composite group and an aeromonas caviae composite group, and is low in cost and high in detection accuracy rate. According to the technical scheme, the detection kit for distinguishing the three aquatic source aeromonas composite groups comprises the following identification materials: 1) a reagent A, sterile water, 2) an identification bottle 1, salt-free alkaline peptone water, 3) an identification bottle 2, RS culture medium containing ampicillin, 4) an identification bottle 3, tryptone soya culture medium, 5) an identification bottle 4, arabinose culture medium, 6) an identification bottle 5, esculoside culture medium, 7) an identification bottle 26, glucose gas medium, and 8) an identification bottle 7, an oxidase strip. The detection kit belongs to the technical field of detection.

Description

technical field [0001] The present invention relates to a detection kit, more specifically, a test kit for distinguishing three common aquatic source aeromonas, Aeromonas hydrophila complex, Aeromonas temperatus complex, and Aeromonas caviae complex. Bacteria complex detection kit. Background technique [0002] Aeromonas belongs to Aeromonas family, widely distributed in water environment, and often detected in human body, aquatic animals and food. Aeromonas derived from aquatic animals is divided into 3 complex groups, including Aeromonas hydrophila complex, Aeromonas temperate complex, and Aeromonas caviae pig complex, mainly including Aeromonas hydrophila, Aeromonas temperate Monomonas, Aeromonas vickerii, Aeromonas caviae, etc., are also common pathogenic bacteria in humans and fish, which can cause food poisoning, diarrhea, and sepsis in humans. Aeromonas has a wide range of pathogenicity. In the high temperature season, it is easy to infect a variety of aquatic anima...

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Problems solved by technology

[0003] At present, the technologies for distinguishing the Aeromonas complex group of aquatic products include: 1. After cultivating and purifying a single colony in a common medium, use a rapid automatic identification system for identification and identification of bacteria. This method requires expensive instruments and equipment and skilled operators; 2. After cultivating and purifying a single bacterium in an ordinary medium, the detection technology using nucleic acid includes DNA and PCR technology (polymerase chain reaction technology). Although it is fast, concise and sensitive, this method requires expensive instruments and equipment and Skilled operators; 3. The classic physiological and biochemical identification method is not only cumbersome, time-consuming, but also requires operators to have good professional quality; 4. At present, there are also some commercial Aeromonas culture medium, such as RS Culture medium, AHM medium and APM medium, etc., but the separation rate and accuracy rate are not high (Ling Hongli, et al., Comparison of the Differentiation Effects of Aeromonas hydrophila Selective Medium, 1998)

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