Extraction method of grape peel RNA (ribonucleic acid)

Abstract

The invention discloses an extraction method of grape peel RNA (ribonucleic acid), belonging to the technical field of biology. The invention aims to provide an extraction method of grape peel RNA, which has the advantages of lower cost and higher efficiency. The method comprises the following steps: weighing 0.2g of grape peel, grinding with liquid nitrogen, adding a preheated lysis solution and beta-mercaptoethanol, whirling, putting in a water bath, adding isometric chloroform / isoamyl alcohol (24 / 1), vibrating, centrifugating, taking the supernatant, adding isometric chloroform / isoamyl alcohol (24 / 1), centrifugating, repeating two times, adding a precipitant, precipitating, centrifugating, washing with ethanol, and recovering the RNA.

Description

technical field [0001] The invention relates to a method for extracting grape peel RNA, which belongs to the field of biotechnology. Background technique [0002] Whether high-quality RNA can be extracted is one of the key basic tasks in the research of molecular biology and functional genomics of fruit trees, such as RT-PCR, Northern hybridization, cDNA library construction, etc., all require high-quality RNA. The high-throughput sequencing technology and high-performance bioinformatics analysis technology developed in recent years have extremely high requirements on the quality of RNA, which makes RNA extraction have higher requirements in terms of integrity and purity. Due to the strong RNase activity, it is relatively difficult to obtain non-degraded RNA. Plant cells have a hard cell wall, large vacuoles and inclusions in the cells, and polysaccharides, polyphenols and other secondary metabolites that are close to the polarity of nucleic acids, making the extraction of ...

AI Technical Summary

Problems solved by technology

[0003] The development and ripening mechanism of grape berries and the regulation mechanism of anthocyanin content in pericarp are one of the focuses of grape molecular biology research, but RNA extraction in its tissues has always been difficult

Due to the high content of polysaccharides and polyphenols in grape peels, some of their physical and chemical properties are similar to RNA, and RNA is easily taken away during the removal of polysaccharides and polyphenols. The existing methods are suitable for the extraction of RNA from leaves, flowers and other tissues. method, not suitable for grape berries, it is difficult to separate them in practice, resulting in a decrease in RNA yield

These problems directly affect the bioinformatics analysis of RNA, and it is difficult to meet the needs of further molecular biology research.

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