High efficient and fast culture method of anoectochilus roxburghii tissue cultured seedling
A fast technology for tissue culture seedlings, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of difficult to ensure seedlings of wild golden clematis, increased pollution rate of tissue cultured seedlings, and many transfer times, etc. problem, to achieve the effects of rapid propagation of clematis, solving the pollution of tissue culture seedlings, and reducing the number of transfers
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[0021] Below in conjunction with embodiment the method of the present invention is further described.
[0022] A method for efficiently and rapidly propagating tissue-cultured seedlings of Clematis clematis, the specific implementation is as follows:
[0023] 1. Cut a small part of the top of the clematis capsule with a blade, wash the surface with clean water, place it in a tissue culture bottle, and then soak it in alcohol with a volume concentration of 75% for 30 seconds in a sterile operating table, and then use a concentration of Soak in 0.1% mercuric chloride aqueous solution for 8 minutes, and finally rinse with sterile water for 3 to 5 times to complete the entire disinfection step.
[0024] 2. Preparation of seed medium:
[0025] (1) Medium composition: + 6-carbylpurine 2.5mg / L~3mg / L+ naphthaleneacetic acid 0.5mg / L~1mg / L+ white sugar 30g / L+ potatoes 200g / L+ agar 5.1g / L;
[0026] (2) Heat the prepared medium until the agar is completely dissolved, and evenly distribu...
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