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Protein GmZF 392 derived from soybeans and applications of related biomaterials in regulation and control of plant fat

A plant and protein technology, applied in the application field of regulating vegetable oils, can solve problems such as little known

Active Publication Date: 2014-11-19
INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in plants, the regulatory mechanism of lipid synthesis and its related genes are still poorly understood

Method used

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  • Protein GmZF 392 derived from soybeans and applications of related biomaterials in regulation and control of plant fat
  • Protein GmZF 392 derived from soybeans and applications of related biomaterials in regulation and control of plant fat
  • Protein GmZF 392 derived from soybeans and applications of related biomaterials in regulation and control of plant fat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067]Example 1, cDNA cloning of the GmZF392 encoding gene related to regulation of soybean lipid metabolism and construction of plant expression vector

[0068] 1. Cloning of protein GmZF392 gene and construction of plant expression vector

[0069] Total RNA was extracted from Heinong 44 seedlings, and the RNA was reverse-transcribed with reverse transcriptase to synthesize cDNA.

[0070] According to the information of GmZF392 full-length cDNA sequence in the soybean genome sequence of PlantGDB, design primers, the primer sequences are as follows:

[0071] GmZF392-up: 5'-ATGAGCAGTGTCTGTGCCA-3';

[0072] GmZF392-dp: 5'-TTACATCAGCAACTCGTTCACC-3'.

[0073] Using HN44cDNA as a template and using GmZF392-up and GmZF392-dp as primers, PCR amplification was performed to obtain a PCR product of about 1Kb. After sequencing, the PCR product is 1179bp, its nucleotide sequence is SEQ ID No.1, the DNA molecule shown in the nucleotide sequence is the GmZF392 gene (GmZF392), the protein...

Embodiment 2

[0082] Embodiment 2, the acquisition of transgenic GmZF392 Arabidopsis

[0083] 1. Obtaining recombinant Agrobacterium

[0084] The recombinant vector pGWB412-GmZF392 containing GmZF392 obtained in Example 1 was introduced into Agrobacterium tumefaciens GV3101 by electroporation to obtain recombinant Agrobacterium GV3101 / GmZF392 containing pGWB412-GmZF392.

[0085] 2. Obtaining and identification of transgenic GmZF392 Arabidopsis

[0086] The recombinant Agrobacterium GV3101 / GmZF392 was cultivated to the logarithmic phase, and then transformed into Arabidopsis thaliana (col-0) by vacuum method. The seeds were purchased from Arabidopsis Biological Resource Center (ABRC). Seeds harvested after cultivation (T 1 generation), the seeds were sown on the MS selection medium containing kanamycin (50mg / L), and the T 1 When the generation plant grows to 4-6 leaves, it is moved to the vermiculite to grow, and the harvest T 1 The seeds of each individual plant were sown separately, an...

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Abstract

The invention discloses a protein GmZF 392 derived from soybeans and applications of related biomaterials in regulation and control of plant fat. One application is a method for cultivation of transgenic seed plants with a high total fat content and / or a high fatty acid content. The method comprises steps: a GmZF 392 gene is introduced into an acceptor seed plant and a transgenic seed plant with a total fat content being higher than the total fat content of the acceptor seed plant and / or a fatty acid content being higher than the total fatty acid content of the acceptor seed plant is obtained. The fatty acids are at least one of the followings: linolenic acid, linoleic acid, oleic acid, a fatty acid named after gongduosuan, sinapic acid, palmitic acid and stearic acid. The GmZF 392 encoding gene has important theoretical and practical significances to raise and improve crop fat components, especially to raise fat components in seeds of oil plants of soybeans and the like and to cultivate varieties with high fat.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of protein GmZF392 derived from soybean and related biological materials in regulating vegetable oil. Background technique [0002] 71% of the fat in the human diet comes from plants. Among several major oil-producing crops in the world, the total oil production of soybean accounts for about 30%, ranking first in the world's vegetable oil production (Table 1). [0003] Table 1. Major oil-producing crops in the world [0004] [0005] The synthesis of fatty acids is one of the most important metabolic pathways in plants. It exists in any cell of plants and is necessary for growth and development. Blocking it leads to cell death, so a plant mutant that blocks fatty acid synthesis has not been found so far. [0006] Plants differ greatly from other eukaryotes in the enzymes involved in the fatty acid synthesis pathway. Synthesis of fatty acids with 16 or 18 carbon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/82A01H5/00
Inventor 陈受宜张劲松李擎天宋庆鑫张万科马彪林晴何锶洁
Owner INST OF GENETICS & DEVELOPMENTAL BIOLOGY CHINESE ACAD OF SCI
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