Antagonistic bacterial strain for preventing and treating fruit tree rot and application thereof
A technology of rot disease and antagonistic bacteria, applied in the field of Bacillus pumilus, can solve the problems of destroying the ecological environment, incompleteness, and high cost of control, and achieves the effect of a wide range of adaptations
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Embodiment 1
[0043] Example 1 Isolation, screening and identification of antagonistic bacteria strain Bacillus pumilusXJAU-117
[0044] 1) Isolation of antagonistic bacteria strain Bacillus pumilusXJAU-117
[0045] The bacterial strain Bacillus pumilusXJAU-117 which antagonizes the fruit tree rot pathogen of the present invention is collected from the Aksu area of Xinjiang. During the process of isolating the walnut tree rot pathogen, the conventional tissue separation method is adopted to sample from the border of the lesion at the junction of the disease and the health. Separated to get:
[0046] 1A) Select three 5×5mm plant tissue blocks with diseased spots, and disinfect the surface with 75% ethanol and 3% NaClO (put the tissue blocks in 75% ethanol, take them out quickly, and then place them in 3% NaClO for disinfection 5 min), and then washed 3 times with sterile water, blotted the water on the surface of the tissue block with sterilized filter paper, placed the 3 tissue blocks ev...
Embodiment 2
[0065] Example 2 Effect of antagonistic bacteria strain Bacillus pumilusXJAU-117 on the growth of rot pathogen
[0066] 1. Determination of the antibacterial rate of the antagonistic bacteria strain Bacillus pumilusXJAU-117 on the pathogenic bacteria of rot
[0067] The inhibitory effect of antagonistic bacteria strain Bacillus pumilusXJAU-117 on walnut rot pathogen, apricot rot pathogen, apple rot pathogen and poplar rot pathogen was detected by plate confrontation method. The assay method is the same as the method in step 2 in Example 1.
[0068] Walnut rot pathogen strains, apricot rot pathogen strains, apple rot pathogen strains, and poplar rot pathogen strains were provided by the Plant Pathology Laboratory of the College of Forestry and Horticulture, Xinjiang Agricultural University. The measurement results are as follows Figure 3-6 and shown in Table 2.
[0069] As shown in Table 2, the strain XJAU-117 has the highest inhibitory rate of 85.88% on walnut tree rot, an...
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