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Preparation method and dyeing method of red pigment secreted by ginkgo leaf endophyte

A dyeing method and endophyte technology, applied in the field of light chemical industry, can solve the problems of poor soaping fastness of dyed fabrics, no reports, etc., and improve the K/S value of dyeing, soaping fastness, and cell growth rate. Accelerate and realize the effect of industrialization

Inactive Publication Date: 2014-10-22
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Cheng Wanli and others studied the application of monascus pigments on silk fabrics, but the soaping fastness of dyed fabrics is poor
[0006] There are no reports on the preparation of red pigment secreted by endophytes of Ginkgo biloba and its application in textile dyeing

Method used

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Examples

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Effect test

preparation example Construction

[0021] A preparation method for red pigment secreted by ginkgo leaf endophytes, specifically comprising the steps of: taking the endophytes of ginkgo leaves and washing the spores with sterile physiological saline, adding physiological saline to the spores, and adjusting the concentration of the spores to 10 4 ~10 5 CFU / ml, then inoculate the adjusted spore suspension in the PDA medium with 1% inoculation amount (ie 100ml culture solution inoculation 1ml) for cultivation, at a temperature of 30°C to 35°C and a pH value of 5 to 7 Under certain conditions, culture for 7-14 days; after the culture is over, remove the cells by suction filtration to obtain a solution containing the red pigment secreted by endophytes of Ginkgo biloba, which can be used for dyeing wool fabrics and modified silk fabrics.

[0022] Based on the dyeing method of the red pigment secreted by a kind of ginkgo biloba endophyte, it is used to dye the dyed object. The dyed object includes wool fabric and silk ...

Embodiment 1

[0029] 10 liters of PDA medium with a concentration of Ginkgo biloba endophytes of 10 4 ~10 5 The spore suspension inoculation amount of CFU / ml is 1%, that is, 100ml, the temperature is 35° C., pH=5, static culture for 7 days, and the red pigment aqueous solution secreted by Ginkgo biloba endophytes is obtained after suction filtration.

Embodiment 2

[0031] 10 liters of PDA medium with a concentration of Ginkgo biloba endophytes of 10 4 ~10 5 The spore suspension inoculation amount of CFU / ml is 1%, that is, 100ml, the temperature is 32° C., pH=6, static culture for 14 days, and the red pigment aqueous solution secreted by Ginkgo biloba endophytes is obtained after suction filtration.

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Abstract

The invention relates to the field of light chemical industry, and aims to provide a preparation method and dyeing method of a red pigment secreted by ginkgo leaf endophyte. The preparation method comprises the following steps: washing ginkgo leaf endophyte spores, adding normal saline to prepare a spore suspension, inoculating in a PDA (potato dextrose agar) culture medium, culturing, and carrying out vacuum filtration after the culture is finished, thereby obtaining the solution containing the red pigment secreted by the ginkgo leaf endophyte. The dyeing method comprises the following steps: taking the solution containing the red pigment secreted by ginkgo leaf endophyte, diluting with water to obtain a dye solution, impregnating the dyed substance in the dye liquor to perform dyeing treatment, taking out the dyed substance after the dyeing is finished, cleaning and drying. The obtained novel natural dye is safe and environment-friendly. The red pigment has the advantages of favorable water solubility and higher substantivity for protein fibers. The fungus pigment is free from the limits of region and environment, and has favorable reproducibility. The biological culture method greatly enhances the yield, and is easies for implementing industrialization.

Description

technical field [0001] The invention relates to the field of light chemical industry, in particular to a preparation method of red pigment secreted by ginkgo biloba endophytes and a dyeing method thereof. Background technique [0002] With the enhancement of people's awareness of environmental protection and concern for their own health, natural dyes are getting more and more attention. Natural dyes are favored by more and more consumers because they do not cause pollution during the preparation process, are biodegradable, have good compatibility with the environment, are rich in sources, are safe to wear on dyed fabrics, have natural hues, and some have good pharmacological effects. [0003] Natural dyes are vegetable dyes. Plant growth is affected by environmental factors such as seasons, climates, and places of origin. It is easy to cause shortage of raw materials, complex extraction process, high cost, and poor dyeing reproducibility. Therefore, it has not been widely us...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/02D06P1/34D06P3/14D06P3/04C12R1/645
Inventor 余志成李红艳南旭莹程荣煌
Owner ZHEJIANG SCI-TECH UNIV
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