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Preparation and application of nitrogen-doped graphene quantum-dot two-photon fluorescence

A technology of graphene quantum dots and nitrogen doping, which is applied in the field of preparation of two-photon fluorescent probes, and achieves the effect of simple preparation method

Inactive Publication Date: 2014-10-22
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the two-photon fluorescence properties of graphene quantum dots and the use of graphene quantum dots as two-photon fluorescent probes for biological imaging have not been reported yet.

Method used

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  • Preparation and application of nitrogen-doped graphene quantum-dot two-photon fluorescence
  • Preparation and application of nitrogen-doped graphene quantum-dot two-photon fluorescence
  • Preparation and application of nitrogen-doped graphene quantum-dot two-photon fluorescence

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Preparation of nitrogen-doped graphene quantum dots

[0043] The powdered graphite flakes used in the present invention are purchased from alfa aser, 325 mesh.

[0044] Heat 15ml of concentrated sulfuric acid to 90°C, add 2.5g of K 2 S 2 O 8 , 2.5g P 2 O 5 , and 3g of powdered graphite flakes reacted for 4.5 hours. After the reaction, the product was diluted with 500 ml of deionized water, filtered, washed, and then placed in a vacuum drying oven. Add 120ml of concentrated sulfuric acid to the dried pretreated graphite oxide, stir evenly, slowly add 15g potassium permanganate in an ice bath at 0°C, remove the ice bath after the system is stable, change to a water bath, and stir at 35°C 2h. Then change to an ice bath, slowly add 1000ml of water for dilution, the solution turns purple-black, after stirring at room temperature for 2h, drop 20ml of 30% H with a glass dropper 2 O 2 , the obtained product was centrifuged to remove the supernatant, washed with 10% HCl...

Embodiment 2

[0048] Preparation of nitrogen-doped graphene quantum dots

[0049] Heat 15ml of concentrated sulfuric acid to 90°C, add 2.5g of K 2 S 2 O 8 , 2.5g P 2 O 5 , and 3g of powdered graphite flakes reacted for 4.5 hours. After the reaction, the product was diluted with 500 ml of deionized water, filtered, washed, and then placed in a vacuum drying oven. Add 120ml of concentrated sulfuric acid to the dried pretreated graphite oxide, stir evenly, slowly add 15g potassium permanganate in an ice bath at 0°C, remove the ice bath after the system is stable, change to a water bath, and stir at 35°C 2h. Then change to an ice bath, slowly add 1000ml of water for dilution, the solution turns purple-black, after stirring at room temperature for 2h, drop 20ml of 30% H with a glass dropper 2 O 2 , the obtained product was centrifuged to remove the supernatant, washed with 10% HCl to completely remove sulfate, and then washed with deionized water until neutral, and finally the obtained s...

Embodiment 3

[0053] Preparation of nitrogen-doped graphene quantum dots

[0054] Heat 15ml of concentrated sulfuric acid to 90°C, add 2.5g of K 2 S 2 O 8 , 2.5g P 2 O 5 , and 3g of powdered graphite flakes reacted for 4.5 hours. After the reaction, the product was diluted with 500 ml of deionized water, filtered, washed, and then placed in a vacuum drying oven. Add 120ml of concentrated sulfuric acid to the dried pretreated graphite oxide, stir evenly, slowly add 15g potassium permanganate in an ice bath at 0°C, remove the ice bath after the system is stable, change to a water bath, and stir at 35°C 2h. Then change to an ice bath, slowly add 1000ml of water for dilution, the solution turns purple-black, after stirring at room temperature for 2h, drop 20ml of 30% H with a glass dropper 2 O 2 , the obtained product was centrifuged to remove the supernatant, washed with 10% HCl to completely remove sulfate, and then washed with deionized water until neutral, and finally the obtained s...

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Abstract

The invention discloses a nitrogen-doped graphene quantum dot, a preparation method thereof and application thereof as a two-photon fluorescence probe to imaging of living cells. The nitrogen-doped graphene quantum dot is simple in preparation method, is good in imaging effect when applied to two-photon fluorescence imaging of living cells, and has extremely strong two-photon fluorescence in a relatively wide pH scope, and the two-photon absorption cross section can reach 48000 GM; the nitrogen-doped graphene quantum dot is extremely easy to dissolve in water, free of toxicity and good in light stability; and a solution of the nitrogen-doped graphene quantum dot has extremely strong stability, is capable of keeping stable within 24 months, and has wide application prospect.

Description

technical field [0001] The invention relates to a preparation method and application of a two-photon fluorescent probe, in particular to a preparation method of a nitrogen-doped graphene quantum dot two-photon fluorescent probe and its application in live cell imaging. Background technique [0002] Two-photon fluorescence microscopy has the characteristics of near-infrared excitation, deep penetration of excitation light into biological tissues, low phototoxicity, and weak spontaneous background fluorescence of organisms, which is significantly better than traditional single-photon fluorescence microscopy. , is one of the most important and powerful tools in the field of life science research. The emergence of laser scanning confocal two-photon fluorescence microscopy has promoted the development of two-photon fluorescence microscopy imaging technology, which has shown excellent application prospects in the field of living biological tissue imaging, and has attracted more an...

Claims

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Application Information

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IPC IPC(8): C09K11/65G01N21/64C12Q1/02
Inventor 宫建茹刘倩
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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