Citric acid aspergillus niger seed continuous culture method based on mycelium pellet dispersion technology

Abstract

A citric acid aspergillus niger seed continuous culture method based on a mycelium pellet dispersion technology comprises the steps as follows: (1), an aspergillus niger spore solution is inoculated to a seed culture medium and cultured and prepared into a mature seed solution; (2), the mature seed solution is treated by a disperser to obtain a dispersed mycelium seed solution; (3), part of the dispersed mycelium seed solution obtained in the step (2) is transferred to a fermentation medium for fermentation with 5%-15% of inoculum concentration, and the fermentation is finished when the reducing sugar concentration of the fermentation medium is lower than 0.5%; and (4), part of the dispersed mycelium seed solution obtained in the step (2) is inoculated to a seed culture medium with 5%-15% of inoculum concentration to be cultured and prepared into a next level of mature seed solution, and then the operation returns to the step (2), so that the continuous culture for aspergillus niger seeds is realized. According to the aspergillus niger seed continuous culture method provided by the invention, the tedious culture process of aspergillus niger spores can be directly reduced, simultaneously, the culture time of mature seeds can be effectively shortened, and the utilization rate of raw materials is increased.

Description

technical field [0001] The invention relates to the technical field of fermentation engineering, in particular to a continuous cultivation method for Aspergillus niger citrate seeds based on mycelium ball dispersion technology. Background technique [0002] Citric acid (Citric Acid) also known as citric acid, chemical name is 2-hydroxypropanetricarboxylic acid, colorless crystal, often contains a molecule of crystal water, odorless, has a strong sour taste, soluble in water, in cold water It is easier to dissolve in water than hot water and is often used for identification and separation. It has a wide range of uses in food, cosmetics, medicine, construction, printing and dyeing, washing and other industries. Because of its pleasant sour taste, refreshing taste, no aftertaste, safety and non-toxicity, it has become the most produced and consumed food in the world. organic acids. The demand for citric acid in emerging industries is showing a gradual increase. Citric acid h...

AI Technical Summary

Problems solved by technology

The traditional seed culture process uses the inoculation method of Aspergillus niger spores, which requires a large number of mature Aspergillus niger spores, and the preparation process of Aspergillus niger spores is relatively complicated

A batch of mature Aspergillus niger spores needs to be screened on plates, cultivated on inclined planes, cultivated in eggplant bottles, and finally cultured in bran koji tanks. The preparation process is cumbersome, the activity screening is complicated, the workload is large, and the preparation cycle needs to be more than 30 days.

At the same time, the process of cultivating seeds by spore inoculation only requires at least 12 hours for spore germination, so mature seeds take a long time to cultivate

[0004] The traditional step-by-step expansion of Aspergillus niger spores has disadvantages such as long and complicated process, heavy screening workload, long cycle, etc., which consumes a lot of raw material costs and operating costs