Composition containing chlorella extract for the prevention or treatment of liver disorders

A technology for chlorella extract and liver disease, which is applied to medical preparations containing active ingredients, drug combinations, medical raw materials derived from algae, etc., and can solve problems such as the clinical effect of chlorella extract that has not yet been disclosed.

Active Publication Date: 2014-10-08
GANGNEUNG WONJU NAT UNIV IND ACAD COOPERATION GROUP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, the clinical effect of chlorella extract as a drug on liver diseases has not been disclosed

Method used

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  • Composition containing chlorella extract for the prevention or treatment of liver disorders
  • Composition containing chlorella extract for the prevention or treatment of liver disorders
  • Composition containing chlorella extract for the prevention or treatment of liver disorders

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Example 1: Concentrated Ethanol Extract of Freshwater Chlorella

[0052] 500 Ml 99.9% ethanol (Daejeong, Korea) was added to 100 g of concentrated powdered freshwater chlorella and stirred in the dark. Next, the resulting mixture was extracted twice every 12 hours and concentrated under reduced pressure to obtain an ethanol extract. Then, the activity of quinone reductase was measured with quantified concentrated ethanol extracts.

Embodiment 2

[0053] Example 2: Isolation and Purification of Chlorella Using Thin-Layer Chromatography The materials used for the prevention and treatment of liver diseases were identified using thin-layer chromatography (TLC). Chlorella ethanol extracts were separated on TLC. TLC can fractionate organic solvent extracts into non-polar or polar materials, and use eluent (hexane:ethyl acetate:acetone=7:3:0.1) to separate Chlorella ethanol extract and chlorophyll a ( figure 1 ). Chlorophyll a R f The value is 0.8. The quinone reductase activity of the isolated Chlorella ethanol extract fraction was measured, and the results are listed in Table 2. As a result, R in the ethanol extract fraction of Chlorella f The fraction with a value of 0.8 showed the highest activity. The most active ethanol extract fractions of Chlorella were separated on TLC under the same conditions, as a result, the fractions with the same R as chlorophyll a (CH1 and CH2) f Fraction of values. The activity of each...

Embodiment 3

[0054] Example 3: Quinone reductase activity of extracts

[0055] Ethanol extracts and components in the isolated fractions were analyzed for induction of quinone reductase activity. In order to measure the induction of quinone reductase activity by the fractions obtained from Chlorella, experiments were performed using a white rat hepatoma cell line (Hepa1c1c7).

[0056] First, a-MEM (minimum essential medium) / 10% FBS (fetal bovine serum) solution was mixed with hepatocyte culture medium so that the number of cells was 1×10 5 cells / Ml, and 100 μl of the resulting solution was added to a 96-well plate, and then placed in 5% CO 2 and 37°C for 24 hours. After the cells were stabilized, compounds from the extract of Chlorella and fractions obtained from the extract were added at 7 different concentrations in two-fold increments from 3.125 to 200 μg / Ml, and then incubated in 5% CO 2 and 37°C for 24 hours. After the culture was completed, the cells were washed with a PBS (phosp...

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PUM

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Abstract

The present invention relates to a composition comprising chlorella extract for preventing or treating liver diseases. The composition comprising the chlorella extract according to the present invention may be applied to medical supplies and health supplementary food and the like, and the chlorella extract of the present invention may be usefully used as a composition for preventing or treating liver diseases because it shows inhibitory effect on liver function injury in a liver function injured model.

Description

technical field [0001] The present invention relates to a composition for preventing or treating liver diseases containing chlorella extract. Background technique [0002] The liver is a very important organ responsible for various metabolisms, detoxification, decomposition, synthesis and secretion in the body, and in particular, its functions are as follows: First, the liver has the function of controlling energy metabolism, and it can convert all Nutrients are metabolized into raw materials for energy production and supplied to the whole body or stored. Second, the liver has the function of synthesizing, storing and distributing about 2000 kinds of enzymes, albumin, serum protein of coagulation factors, bile acids and lipids such as phospholipids and cholesterol. Third, the liver has the function of secreting various metabolites to the duodenum via the bile duct, and due to its immune function, the liver plays an important role in maintaining life. Finally, due to its de...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/68A61K9/20A61K9/16A61K9/48A61P1/16A61P1/00
CPCA61K9/20A23L1/337A23L1/30A61K36/05A61K31/555A23L1/3002A61K9/0053A23L33/10A23L33/105A23L17/60A61P1/00A61P1/16A61K35/68A61K9/16
Inventor 卞熙国钱重均金世权李廷权
Owner GANGNEUNG WONJU NAT UNIV IND ACAD COOPERATION GROUP
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