Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Molecular marker, primer and application thereof closely linked to maize bacterial wilt resistance gene

A molecular marker and resistance gene technology, applied in the field of agricultural biology, can solve the problems of low selection efficiency and long breeding cycle, and achieve the effect of reducing labor land, shortening breeding cycle, and improving breeding efficiency

Active Publication Date: 2016-04-27
HEFEI FENGLE SEED
View PDF2 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Conventional breeding methods based on phenotypic selection have disadvantages such as low selection efficiency and long breeding cycle. It is urgent to inject modern molecular technology, supplemented by high-efficiency genotype-directed selection, in order to quickly and efficiently breed excellent new varieties of maize.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Molecular marker, primer and application thereof closely linked to maize bacterial wilt resistance gene
  • Molecular marker, primer and application thereof closely linked to maize bacterial wilt resistance gene
  • Molecular marker, primer and application thereof closely linked to maize bacterial wilt resistance gene

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1. Obtaining the nucleotide sequence shown in SEQ ID NO: 3 in the sequence table and the nucleotide sequence shown in SEQ ID NO: 4 in the sequence table of the primer pair dedicated for identification of bacterial wilt resistance traits

[0029] 1. Parental genome amplification

[0030] The parents used to create the corn recombinant inbredline (RIL) population are 1145 and 0908 selected by our company. 0908 is the female parent and is not resistant to bacterial wilt; 1145 is the male parent and highly resistant to bacterial wilt.

[0031] The genomic DNA of the parental leaves was extracted by the CTAB method, and the primer pair published by QingYang (QingYang, ZhiLi, etal. The composition of the nucleotide sequence shown in 6 was commissioned to be synthesized by Beijing Zixi Biological Company, and then amplified. The PCR reaction system is: 10XBuffer 2μl (including Mg2 + ), dNTP 0.4μ1 (10mM), SEQIDNO: 5 shown in the sequence listing and SEQIDNO: 6 shown in the se...

Embodiment 2

[0038] Example 2. Correlation verification between the amplified product of QK1 and corn bacterial wilt resistance traits

[0039] Using high-resistance material 1145 and non-resistance material 0908 as parents, 96 F after the two crossed 2 Substitute materials are the test objects. The genomic DNA of corn endosperm was extracted by alkaline cooking, and the specific steps were the same as in Example 1. The PCR amplification experiment was performed with QK1. The PCR reaction system is: the PCR reaction system is: 10XBuffer 2μ1 (including Mg2+), dNTP 0.4μ1 (1OmM), the nucleotide sequence shown in SEQIDNO: 3 in the sequence table and the nucleotide sequence shown in SEQIDNO: 4 in the sequence table are each 2μ1 (5μM), 1μ1 of genomic DNA extracted from corn to be tested, 0.5μ1 of taq enzyme (5U / μ1), reaction volume of 20μ1, and a drop of mineral oil to cover. The PCR reaction program is: 94℃5min; 94℃60s, 53.5℃60s, 72℃60s, 35 cycles; 72℃10min.

[0040] The PCR amplification product...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a molecular marker which is closely linked with corn bacterial wilt resistance genes. The molecular marker is a nucleotide sequence as shown in SEQ ID NO.1 in a sequence table or a nucleotide sequence as shown in SEQ ID NO.2 in the sequence table. A primer pair for amplifying the molecular marker is a nucleotide sequence as shown in SEQ ID NO.3 in the sequence table and a nucleotide sequence as shown in SEQ ID NO.4 in the sequence table. When the molecular marker is applied to corn bacterial wilt detection, amplification is performed through PCR, if an obtained PCR amplification product is a 234bp basic group fragment, the corn to be detected is of a bacterial wilt resistant variety, and if the obtained PCR amplification product is a 274bp alkali group fragment, the corn to be detected is of a bacterial wilt infected variety. By adopting the method, whether the corn has the bacterial wilt resistance or not can be detected before the corn is seeded, selective seeding can be achieved, the breeding time is shortened, and the method is rapid, simple and convenient and can be widely applied to assisted breeding of corn.

Description

Technical field [0001] The invention belongs to the field of agricultural biotechnology, and specifically relates to a method for detecting corn bacterial wilt resistance, PCR primers and applications thereof. Background technique [0002] Conventional breeding methods based on phenotypic selection have shortcomings such as low selection efficiency and long breeding cycles. It is urgent to inject modern molecular techniques, supplemented by high-efficiency genotype-directed selection, to quickly and efficiently breed excellent new maize varieties. With the rapid development of molecular biology and genomics, molecular marker technology has become more widely used. PCR-based molecular markers such as microsatellites or SSR (simplesequence repeat) have the characteristics of high polymorphism rate, relative stability, simple and rapid detection methods and easy operation, and are widely used. Since molecular marker-assisted selection is not susceptible to environmental factors and...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11C12Q1/68A01H1/04
Inventor 朱先飞耿延琢王利明王兆贤张二朋杨焰华齐伟王丽梅
Owner HEFEI FENGLE SEED
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com