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A kind of domestic sludge reduction composite microbial preparation and its preparation and application method

A technology of composite microorganisms and domestic sludge, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems that the composite microbial agent does not conform to physical sludge, does not conform to the concept of clean production, and has large funds. To achieve the effect that is conducive to rapid proliferation

Active Publication Date: 2017-12-15
ANHUI QINGMING ENVIRONMENTAL PROTECTION TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there are two problems of effectiveness and economy in the treatment and disposal of excess sludge: first, there is no effective method that can be generalized and has no pollution to the environment; second, various sludge treatment and Disposal method requires huge capital
The limitation of its technology is that the composite bacterial agent does not conform to the characteristics of physical sludge, and this sludge terminal reduction technology does not conform to the concept of clean production

Method used

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  • A kind of domestic sludge reduction composite microbial preparation and its preparation and application method
  • A kind of domestic sludge reduction composite microbial preparation and its preparation and application method
  • A kind of domestic sludge reduction composite microbial preparation and its preparation and application method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] The first step: scale up and cultivate separately

[0043] Cellulomonas CCTCC AB209393 was activated by a slope, and then inoculated into an Erlenmeyer flask containing LB liquid medium and cultured at 30°C for 36 hours. The above-mentioned cultured bacteria liquid was connected to the seed tank at 5% inoculum amount, the aeration volume of the seed tank was 1:0.5, the culture temperature was 30℃, the stirring speed was 120rpm, the culture time was 48h, and it was cultured to the logarithmic growth phase; After the bacterium CICC20143 was activated on the slope, it was inoculated into an Erlenmeyer flask containing LB liquid medium and cultured at 30°C for 48 hours. The above-mentioned cultured bacteria solution was connected to the seed tank at 5% inoculation amount. The air volume of the seed tank was 1:1.5, the culture temperature was 30°C, the stirring speed was 150 rpm, and the culture time was 60 hours; Bacillus pumilus ACCC10113 was inoculated after the slope activa...

Embodiment 2

[0047] The first step: scale up and cultivate separately

[0048] Cellulomonas CCTCC AB209393 was activated by a slope, and then inoculated into an Erlenmeyer flask containing LB liquid medium and cultured at 37°C for 48 hours. The above-mentioned cultured bacteria liquid was connected to the seed tank at 5% inoculum amount, the aeration volume of the seed tank was 1:0.5, the culture temperature was 30℃, the stirring speed was 150rpm, the culture time was 72h, and it was cultured to the logarithmic growth phase; After the bacterium CICC20143 was activated on the inclined plane, it was inoculated into an Erlenmeyer flask containing LB liquid medium and cultured at 37°C for 48 hours. The above-mentioned cultured bacteria solution is connected to the seed tank at a 10% inoculum amount. The aeration volume of the seed tank is 1:1.5, the culture temperature is 30°C, the stirring speed is 160rpm, the culture time is 60h, and it is cultured to the logarithmic growth phase; Bacillus pumi...

Embodiment 3

[0052] The first step: scale up and cultivate separately

[0053] After being activated by the incline, it was inoculated into an Erlenmeyer flask containing LB liquid medium and cultured at 30°C for 48 hours. The above-mentioned cultured bacteria solution is connected to the seed tank at a 10% inoculum amount, the air volume of the seed tank is 1:1.5, the culture temperature is 30℃, the stirring speed is 160rpm, the culture time is 60h, and it is cultivated to the logarithmic growth phase; After CICC31192 is activated on the slope, it is inoculated into a YPD liquid medium containing Erlenmeyer flask and incubated at 28°C for 56 hours. Connect the above-mentioned cultured bacteria liquid to the seed tank at a 5% inoculum amount. The aeration volume of the seed tank is 1:1.5, the culture temperature is 30°C, the stirring speed is 120rpm, the culture time is 48h, and it is cultured to the logarithmic growth phase; live bacteria count , To detect the number of bacteria, mix accord...

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Abstract

The invention relates to a household sludge reduction compound microbial preparation as well as preparation and application methods thereof, belonging to the field of microbial application. Based on the total bacteria count, the household sludge reduction compound microbial preparation comprises 10-20% of Celloulonmonassp., 10-20% of Serratiasp., 30-60% of Bacilluspumilus and 20-30% of Rhodotorulamucilaginosa, wherein the Celloulonmonassp. is selected from at least one of CCTCCAB209393 and YMCCKMS3990, the Serratiasp. is selected from at least one of ACCC02490 and CICC20143, the Bacilluspumilus is ACCC10113 and the Rhodotorulamucilaginosa is CICC31192. The microbial preparation and the preparation method provided by the invention are simple and feasible, economic and reasonable and free from secondary pollution to the environment.

Description

Technical field [0001] The invention provides a composite microbial preparation for reducing domestic sludge and a preparation and application method thereof, belonging to the field of microbial applications. Background technique [0002] As the scale of cities expands and the population increases, the amount of sewage treatment increases year by year. As of the end of June 2013, a total of 3,479 urban sewage treatment plants have been built in cities and counties nationwide, and there have been sewage treatment plants in 650 cities across the country, accounting for 98.9% of the total number of cities with municipalities, forming a sewage treatment capacity of 1.2 ×108m 3 / d. [0003] At present, the activated sludge method is a common method of sewage treatment. While the effluent reaches the standard, it is accompanied by a large amount of excess sludge with high moisture content. The moisture content of the remaining sludge is generally above 95%, and the content of organic m...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N1/16C02F11/02C12R1/425C12R1/07C12R1/645C12R1/01
Inventor 赵海泉张龙胡子全汪顺丽宋永庆张宏才柴化建魏荷芬
Owner ANHUI QINGMING ENVIRONMENTAL PROTECTION TECH CO LTD
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