Method for performing regeneration propagation on sterile stalks of butterfly orchids
A technology of phalaenopsis and pedicel, which is applied in the field of seedling cultivation, can solve the problems of poor ornamental value of native species, achieve the effect of reducing production cost and huge sales volume
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Embodiment 1
[0014] (1) Cut the Phalaenopsis pedicels with side buds without pests and diseases into 2-3cm segments, remove the bracts on the outer layer of the buds with tweezers and other tools, add 4-6ml Tween 20 and stir well, then pour in tap water Rinse under water for more than 30 minutes, transfer to the ultra-clean workbench and disinfect with 70% to 75% alcohol for 30 seconds, then treat with sterile water for 2 to 3 times, add 2% NaClO solution for 5 to 6 minutes, and then rinse with sterile water for 4 ~5 times, use sterile filter paper to blot the surface moisture, cut off the surface of the flower stalk that is in contact with the disinfectant, and inoculate it into the primary culture medium for cultivation. The primary medium is: the above-mentioned step (1) The primary medium is: 1 / 2MS +5.0mg / L6-BA+0.5mg / LNAA+20g / L sucrose+6 / L agar+400mg / L citric acid+40g / L banana puree, pH value is 5.8, culture condition The method is: temperature 25±2°C, dark culture for 7-10 days in ...
Embodiment 2
[0019] The difference with embodiment 1 is:
[0020] The primary medium in the step (1) is: 1MS +5.0mg / L6-BA+0.5mg / LNAA+20g / L sucrose+6 / L agar+400mg / L vitamin C+40g / L potato juice, The pH value is 5.8.
Embodiment 3
[0022] The difference with embodiment 1 is:
[0023] The primary medium in the step (1) is: 1 / 2MS + 3mg / L6-BA + 0.3mg / LNAA + 25-30g / L sucrose 6.5g / L agar + 350mg / L citric acid + 35g / L Banana puree; the subculture medium in the step (2) is: 1 / 2MS +5mg / L6-BA+0.4mg / LNAA+25~30g / L sucrose+6.5g / L agar+400mg / L activated carbon +35g / L coconut milk; the rooting medium in the step (3) is: 1 / 2MS+1.5mg / L NAA+30g / L sucrose+6 g / L agar+3g / L activated carbon+40g / L Mashed bananas; pH 5.6.
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