Method for detection of thrombin in the concentration range of 0.5-10 μg/mL using carbon nanotube microcantilever biosensor

Abstract

A carbon nanotube microcantilever biosensor is used to detect thrombin with a concentration range of 0.5-10µg / mL, which is realized by constructing a carbon nanotube microcantilever biosensor. The biosensor includes a support, a base material, a carbon nanotube, and a pick-up circuit, and a layer of nucleic acid aptamer is modified on the carbon nanotube through π-π superposition. First, a detection probe containing a thrombin nucleic acid aptamer is made on the carbon nanotube micro-cantilever beam. During detection, the detection probe is put into the sample to be tested, and the thrombin in the sample to be tested reacts with the detection probe through a specific reaction. The nucleic acid aptamer on the microcantilever forms a complex and attaches to the microcantilever; the mass change generated by the complex on the microcantilever causes the flexural displacement or the change of the resonance frequency of the microcantilever and the relationship between the mass size of the complex and the The concentration of thrombin in the sample to be tested is positively correlated, thereby realizing the detection of thrombin.

Description

technical field [0001] The invention relates to the field of biomedical engineering, in particular to a method for detecting thrombin with a micro-cantilever beam biosensor. technical background [0002] Thrombin is a multifunctional serine protease that plays an important role in a series of physiological and pathological processes in vivo. It is not only involved in hemostasis and coagulation, inflammation, immune response, tissue repair and wound healing, but also activates the tumorigenic potential of normal cells and leads to the metastatic phenotype of malignant cells. In addition, thrombin is also a mitogenic agent of tumor cells, which can enhance the proliferative response of tumor cells to cytokines, enhance the adhesion of tumor cells to platelets and the invasion of cell matrix in vitro, and promote tumor angiogenesis and tissue reconstruction of the tumor microenvironment. Therefore, the detection of thrombin is of great clinical significance. At present, thro...

AI Technical Summary

Problems solved by technology

Among them, although the chromogenic substrate method is simple and feasible, its sensitivity is not high, and it is not suitable for microanalysis

However, the fluorescence method has cumbersome operation steps, the fluorescence background is not easy to reduce, and the sensitivity is low.

It is difficult to realize the diagnosis and research of early diseases, and cannot meet the needs of rapid detection

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