Method for preparing diosgenin by virtue of homogenization process synergetic enzymatic hydrolysis

A technology of diosgenin and homogenate method, which is applied in the fields of steroids, fermentation, organic chemistry, etc., can solve the problems of high energy consumption, long production cycle, low extraction rate, etc., to increase the diffusion area and improve the yield , the effect of reducing resistance

Inactive Publication Date: 2014-10-01
NANJING ZELANG MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional method requires repeated extraction and concentration, high energy consumption, long production cycle, low extraction rate, and serious pollution to the environment; while supercritical fluid extraction and ultrasonic extraction are effective, but There is a shortcoming of high one-time investment in equipment
Such as the method disclosed in patent 200410060679.X, its shortcoming is that traditional hydrochloric acid is still used as a catalyst, and a large amount of acidic organic wastewater is produced, which cannot fundamentally solve the pollution problem of diosgenin production, and the yield is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Dry the impurity-removed slices of Pangosia in the sun, dry and crush them, pass through a 100-mesh sieve, take 100g, add 50% ethanol that is 5 times (weight ratio) of the raw materials, use a high-speed homogenizer, and under the power of 200W, process the pangosaurus powder Treat for 8 minutes to obtain dioscin extract, then add 3% (by weight) compound enzyme of raw materials (the ratio of cellulase, hemicellulase and amylase is 0.4:0.2:0.4), and adjust the pH to 3.5 with acetic acid / sodium acetate , stirred evenly, enzymatically hydrolyzed at 50°C for 2h, inactivated at 80°C for 15min, adjusted the pH to neutral with sodium hydroxide, centrifuged, concentrated, and recrystallized from petroleum ether to obtain 1.92g of diosgenin. After testing, the yield of diosgenin was The rate is 1.92%.

Embodiment 2

[0013] Dry the impurity-removed slices of Pangosia in the sun, dry and crush them, pass through a 100-mesh sieve, take 100g, add 70% ethanol that is 7 times (by weight) of the raw materials, use a high-speed homogenizer, and under the power of 200W, process the pangosaurus powder Treat for 10 minutes to obtain dioscin extract, then add 4% (by weight) compound enzyme of raw materials (the ratio of cellulase, hemicellulase and amylase is 0.4:0.2:0.4), and adjust the pH to 4.5 with acetic acid / sodium acetate , stirred evenly, enzymatically hydrolyzed at 55°C for 1.5h, inactivated at 80°C for 15min, adjusted the pH to neutral with sodium hydroxide, centrifuged, concentrated, and ethanol recrystallized to obtain 2.05g of diosgenin. After testing, the yield of diosgenin was The rate is 2.05%.

Embodiment 3

[0015] Dry the impurity-removed slices of Pangosia in the sun, dry and crush them, pass through a 100-mesh sieve, take 100g, add 65% ethanol that is 8 times (weight ratio) of the raw materials, and use a high-speed homogenizer to process the pangosaurus powder at a power of 200W. Treat for 9 minutes to obtain dioscin extract, then add 3.5% (by weight) compound enzyme (the ratio of cellulase, hemicellulase and amylase is 0.4:0.2:0.4) of raw materials, and adjust the pH to 4.0 with acetic acid / sodium acetate , stirred evenly, enzymatically hydrolyzed at 60°C for 1 hour, inactivated at 80°C for 15 minutes, adjusted the pH to neutral with sodium hydroxide, centrifuged, concentrated, and methanol recrystallized to obtain 1.97 g of diosgenin. After testing, the yield of diosgenin was was 1.97%.

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Abstract

The invention relates to a method for extracting diosgenin by virtue of homogenization process synergetic enzymatic hydrolysis. The method comprises the following treatment steps: removing impurities of ningpo yam rhizome, cutting into slices, drying, baking and crushing, screening by using a 100-mesh sieve, adding 50-70% ethanol which accounts for 5-8 times of the weight of a raw material, using a high-speed pulp refiner, treating ningpo yam rhizome powder for 8-10 minutes at a power of 200W to obtain a dioscin extracting solution, and then performing enzymatic hydrolysis, inactivation, centrifugation and re-crystallization to obtain diosgenin. The method disclosed by the invention is used for extracting diosgenin by adopting homogenization process synergetic enzymatic hydrolysis, is simple in process, short in extraction time and high in extraction rate, does not need expensive instruments, is low in production cost, and can be used for industrial production.

Description

technical field [0001] The invention belongs to the field of separation of effective components of natural products, and in particular relates to a method for extracting diosgenin by means of a homogenate method and cooperative enzymatic hydrolysis. Background technique [0002] Pangolin is the dried rhizome of Dioscorea pangolin, which contains diosgenin as the main component. There are more than 60 species of Dioscorea plant resources in my country, most of which contain a large amount of diosgenin. Diosgenin is hydrolyzed by acid or enzyme to generate diosgenin (commonly known as diosgenin), 1 molecule of glucose and 2 molecules of rhamnose. Diosgenin is a white crystal with a melting point of 204-207°C. It is soluble in commonly used organic solvents such as ether, petroleum ether, gasoline and acetic acid, but insoluble in water. It is an important synthetic raw material for the synthesis of various steroid drugs. [0003] At present, there are many patents and litera...

Claims

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Application Information

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IPC IPC(8): C12P33/20C07J71/00
Inventor 刘东锋杨成东
Owner NANJING ZELANG MEDICAL TECH
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