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A strain of Lactobacillus plantarum with the ability to lower cholesterol

A technology of Lactobacillus plantarum and cholesterol, applied in the biological field, can solve the problems of low application value and low degradation rate, and achieve the effects of improving the body's immunity, reducing serum cholesterol, and degrading cholesterol

Active Publication Date: 2016-06-01
哈尔滨美华生物技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In addition, there are reports of cholesterol-lowering lactic acid bacteria isolated from fermented meat, vegetables and other foods, but the degradation rate is not high, and the application value in food health products and medicines is not high

Method used

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  • A strain of Lactobacillus plantarum with the ability to lower cholesterol
  • A strain of Lactobacillus plantarum with the ability to lower cholesterol
  • A strain of Lactobacillus plantarum with the ability to lower cholesterol

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Screening and identification of Lactobacillus plantarum Lp5 with high cholesterol-lowering ability according to the present invention

[0026] 1. Sample source

[0027] It comes from the traditional fermented yak milk product of Guoluo Tibetan Autonomous Prefecture, Qinghai Province, my country.

[0028] 2. Isolation and screening of lactic acid bacteria from traditional yak dairy products

[0029] Take 1ml of fermented yak milk product and dilute it with sterilized physiological saline at a ratio of 1:10, which is 10 -1 dilution. Then draw 1ml of the above diluent, and then dilute it 10 times, which is 10 -2 dilution, and so on. Pick 10 -6 Dilution: Pipette 100ul of the dilution into SL medium, culture anaerobically at 37°C for 48 hours, pick characteristic colonies, and further purify to obtain a pure isolated strain named Lp5.

[0030] 3. Identification of strains

[0031] (1) Gram staining, catalase test

[0032] Gram staining: Pick colonies on the...

Embodiment 2

[0037] Determination and contrast of embodiment 2 cholesterol-lowering ability

[0038] Adjust the number of Lactobacillus plantarum Lp5 to 1.0×10 9 cfu / mL, inoculate 10 ml of MRS liquid medium containing cholesterol 50 mg / ml according to 1% inoculum size, and culture statically at 37°C. Immediately sample 1mL after shaking, centrifuge at 4000rpm / min for 5min, absorb 0.5mL supernatant, and measure the cholesterol content by the o-phthalaldehyde method. After inoculation, the fermented liquid was cultured statically at 37°C for 48 hours, and then the cholesterol content in the supernatant was determined by the same method. The degradation rate of cholesterol was calculated by the following formula:

[0039] Cholesterol degradation rate = (cholesterol content in 0h supernatant - cholesterol content in 48h supernatant) / cholesterol content in 0h supernatant × 100%

[0040] Table 1 Cholesterol-lowering rate of Lactobacillus plantarum Lp5

[0041]

[0042] The cholesterol deg...

Embodiment 3

[0043] Embodiment 3: Digestive tract simulation test

[0044] The selected Lactobacillus plantarum Lp5 was subjected to artificial gastric juice and artificial intestinal juice tolerance experiments.

[0045] Test of tolerance to artificial gastric juice: After 2 generations of cell activation, the seed liquid was centrifuged at 5000r / min for 10 min, washed 3 times with sterilized normal saline, and then suspended. Take 1 mL of the bacterial suspension and put it in artificial gastric juice sterilized by filtration (including 9 mL of artificial gastric juice at pH 3.0), mix well and then culture at a constant temperature of 37°C. Samples were taken at the beginning of the experiment and 3 hours later, and diluted 10 times with sterile saline. Select an appropriate dilution, take 0.1mL of the bacterial suspension and spread it on the MRS plate, and set up 2 repetitions. Count after 48 hours of anaerobic constant temperature cultivation at 37°C. The test results are shown in Ta...

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Abstract

The invention discloses lactobacillus plantarum capable of lowering cholesterol. The lactobacillus plantarum is separated from the traditional fermented yak yoghourt of the Qinghai-Tibet Plateau, and the collection number is CGMCC No.9017. The lactobacillus plantarum can remarkably lower the contents of total cholesterol, triglyceride and low-density lipoprotein, and can be used for preparing fermented diary products, health-care foods and medicines.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the field of development and application of functional lactic acid bacteria, in particular to a strain of Lactobacillus plantarum capable of lowering cholesterol. Background technique [0002] With the continuous improvement of social development and people's living standards, the daily diet structure is also constantly changing. The negative impact of unreasonable dietary structure is that the incidence of cardiovascular and cerebrovascular diseases such as coronary heart disease, hypertension, and atherosclerosis continues to increase. The content of cholesterol in serum is considered to be the main factor in inducing cardiovascular and cerebrovascular diseases. [0003] Cholesterol is an essential nutrient for the human body. For the human body, cholesterol can synthesize a variety of hormones; form cholic acid, promote fat digestion; form cell membranes; and help repair and mai...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/68C12Q1/04A23L33/135A23C9/12A61K35/747A61P3/06C12R1/25A23L33/00
Inventor 郭旭生丁武蓉石超柯文灿保安安陈明
Owner 哈尔滨美华生物技术股份有限公司
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