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Method of synthesizing acrylamide by using acrylonitrile hydratase

A technology for acrylonitrile hydration and acrylamide, applied in the direction of microorganism-based methods, biochemical equipment and methods, and specific-purpose bioreactors/fermenters, can solve the problems of heat transfer in hydration reactions, enzyme inactivation, etc., and achieve Reduce the inhibitory effect, increase the specific surface area, and avoid the effect of excessive local concentration

Active Publication Date: 2014-09-24
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the main problems faced by the microbial method include the enzyme inactivation problem caused by mass transfer and the heat transfer problem of the hydration reaction.

Method used

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  • Method of synthesizing acrylamide by using acrylonitrile hydratase
  • Method of synthesizing acrylamide by using acrylonitrile hydratase
  • Method of synthesizing acrylamide by using acrylonitrile hydratase

Examples

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preparation example Construction

[0027] In the preparation method of acrylamide provided by the present invention, acrylonitrile undergoes a hydration reaction under the catalysis of acrylonitrile hydratase in free cell fluid:

[0028]

[0029] In the above reaction process, because acrylonitrile and the acrylamide produced will inhibit the enzyme activity of acrylonitrile hydratase, experiments have shown that acrylonitrile will inhibit the enzyme activity when the concentration reaches 0.4mol / L. The cell culture medium must be added slowly. Therefore, in the traditional preparation method of acrylamide, the whole reaction takes a long time, and in order to ensure sufficient mixing, strong stirring is often used to increase energy consumption to a large extent; on the other hand, since acrylamide reaches 250g / L also has a more obvious inhibitory effect on the enzyme activity, so the traditional industrial stirred tank reactor can only obtain acrylamide products with a lower concentration in a longer reac...

Embodiment 1

[0034] The dispersed phase used is the oil phase, that is, pure acrylonitrile. The initial continuous phase is TH-3 type Rhodococcus free cell fluid. Take 80mL of free cell solution with an enzyme activity of 2000U, put it into a three-neck flask and stir. The three-neck flask was placed in an ice-water bath to ensure that the temperature of the entire reaction system was not higher than 5°C. A membrane dispersion reactor is used to mix and react acrylonitrile and free cell liquid, the continuous phase is continuously circulated and the dispersed phase is sheared to form monodisperse acrylonitrile droplets, and the catalytic reaction is carried out. After the mixed acrylonitrile and free cell liquid are mixed, the reaction continues through a 1-meter-long coil, and the temperature in the coil is guaranteed not to be higher than 5°C. The reacted mixed solution flows into a three-necked flask to continue to catalyze the acrylonitrile and circulate continuously. 2 mL of the mix...

Embodiment 2

[0036] The dispersed phase used is the oil phase, that is, pure acrylonitrile. The initial continuous phase is TH-3 type Rhodococcus free cell fluid. Take 80mL of free cell solution with an enzyme activity of 2000U, put it into a three-neck flask and stir. The three-neck flask was placed in an ice-water bath to ensure that the temperature of the entire reaction system was not higher than 5°C. A membrane dispersion reactor is used to mix and react acrylonitrile and free cell liquid, the continuous phase is continuously circulated and the dispersed phase is sheared to form monodisperse acrylonitrile droplets, and the catalytic reaction is carried out. After the mixed acrylonitrile and free cell liquid are mixed, the reaction continues through a 1-meter-long coil, and the temperature in the coil is guaranteed not to be higher than 5 oC. The reacted mixed solution flows into a three-necked flask to continue to catalyze acrylonitrile and circulate continuously. 2 mL of the mixtur...

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Abstract

The invention relates to a method of synthesizing acrylamide by using acrylonitrile hydratase. The method comprises the following steps: providing a container which contains a free cell solution; providing a film reactor which comprises a microporous film dividing the film reactor into a first chamber and a second chamber; continuously adding the free cell solution to the first chamber of the film reactor through a first conveying pipeline, wherein the free cell solution flows along the surface of the microporous film in the first chamber; providing acrylonitrile which is continuously added into the second chamber of the film reactor through a second conveying pipeline, wherein acrylonitrile penetrates through the microporous film and enters the first chamber to be added into the flowing free cell solution to be mixed to form a mixed solution, and the mixed solution flows out of the second chamber and enters a reaction pipeline; circularly regurgitating the mixed solution in the reaction pipeline to the container, wherein the solution in the container then circularly flows into the first chamber of the film reactor to circularly react with acrylonitrile penetrating through the microporous film.

Description

technical field [0001] The invention relates to a method for synthesizing acrylamide by using acrylonitrile hydratase. Background technique [0002] As an important chemical raw material, acrylamide is mainly used for the preparation of polyacrylamide. As an excellent flocculant, polyacrylamide can be widely used in petroleum industry, mining industry, coal washing industry, metallurgical industry, water treatment, sugar industry, building materials industry and many other fields. Especially since the use of polyacrylamide in my country is still in the initial stage in the field of papermaking water treatment, with the enhancement of environmental protection awareness and emphasis on water treatment, the market demand for polyacrylamide in my country will further expand. [0003] The traditional preparation methods of acrylamide generally include sulfuric acid hydrolysis and copper-catalyzed hydration. Among them, the sulfuric acid hydrolysis method uses acrylonitrile to r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/02C12M1/00C12R1/01
Inventor 王玉军骆广生李家辉
Owner TSINGHUA UNIV
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