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Plantrennet and application thereof

A plant protease and proteolysis technology, applied in the fields of application, hydrolase, cheese substitute, etc., can solve the problems that have not been reported publicly, and achieve the effects of wide application range, stable enzymatic properties and broad prospects

Inactive Publication Date: 2014-09-24
YUNNAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Through document retrieval, do not see the public report identical with the present invention

Method used

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  • Plantrennet and application thereof
  • Plantrennet and application thereof

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Embodiment Construction

[0014] 1, plant protease of the present invention obtains through the following steps:

[0015] (1) Raw materials and pretreatment: Weigh 10kg of air-dried milk pulp rattan without mildew and dust, cut it off, rinse it quickly with clean water, drain the water, and smash it into 1-2cm 3 particles

[0016] (2) Soaking: Add 200kg of distilled water and soak in a 58°C water bath for 30 minutes.

[0017] (3) Remove impurities: filter with 4 layers of gauze, and then centrifuge the filtrate to remove fine impurities (5000r / min, 15min).

[0018] (4) Concentration: the filtrate is vacuum concentrated in a rotary evaporator to 15% of the original volume (55,°C-0.075MPa), the concentrate is about 30kg, and cooled to 0-4°C.

[0019] (5) Extraction of protease: slowly add ammonium sulfate to the cooling concentrate until the saturation is 20%, aging (4°C, 20min), centrifugation (5000r / min, 5min); take the supernatant and add ammonium sulfate again to saturation The concentration is 60...

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Abstract

The invention relates to plantrennet and the application thereof, and belongs to the technical field of food science. The molecular weight of the plantrennet is 30 KDa and 26 KDa. Sulfydryl is the main functional group of an enzyme. The enzymatic characteristics of the plantrennet are that curd activity and protein decomposition activity are provided, the optimum temperature of the enzyme is 85 DEG C, the optimum protein decomposition activity temperature ranges from 75 DEG C to 80 DEG C, the enzyme activity pH value ranges from 4.0 to 11.0, the optimum enzyme activity pH value is 8.0, oxidation resistance and reducing capacity of the enzyme are weak, and metal ions influence enzyme activity. In mass, 60% of plantrennet, 30% of calcium chloride and 10% of citric acid are mixed to prepare a 0.15% water solution according to the mass ratio, and the water solution is used as a milk coagulant and is applied into cheese processing with the mass accounting for 30% of raw milk mass. The plantrennet has the advantages of being high in yield, good in quality, safe, efficient, stable in enzymatic property, wide in application range, simple in using method, capable of being applied into chess processing and wide in prospect in biological agent development.

Description

Technical field: [0001] The invention relates to a plant protease and its application, belonging to the technical field of food science. Background technique: [0002] Yunnan is a famous kingdom of animals and plants, and a province with strong biological resources is the strategic goal of Yunnan's development. According to the survey, in the northwestern part of Yunnan, there is a natural rattan plant commonly known as "milk milk rattan" (according to the flora of Yunnan, it belongs to the family of Romoaceae and the genus Nanshan rattan, climbing woody rattans, and grows in mountains at an altitude of 500 to 3000 meters. forest or bushes), its stems are crushed and soaked in warm water with an aqueous solution that has the function of curdling milk. Local milk goat farmers use it as a curdling agent to process goat milk cakes. The product has a special fragrance and high nutritional value . Milk cake is one of the three traditional ethnic dairy products in Yunnan (milk c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/50A23C19/04
CPCC12N9/63A23C19/041
Inventor 黄艾祥陶亮张亚丽
Owner YUNNAN AGRICULTURAL UNIVERSITY
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