CRP latex-reinforced immunonephelometry reagent, its kit and use of kit
An immunoturbidimetric and latex-enhanced technology, applied in the field of medical testing, can solve the problems of poor repeatability, antibody inactivation, time-consuming, etc., and achieve the effect of adapting to a wide range of instruments, simple composition, and good stability
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Embodiment 1
[0047] 1. Preparation of assay reagents and kits:
[0048] 1. Dilute the surface carboxylated polystyrene latex particles (103nm) with 50mM pH 6.0 MES buffer to 10ml, the final concentration is 1%;
[0049] 2. Add 200 mg of EDC to the above solution, add 10 mg of CRP antibody from affinity chromatography, and mix at room temperature for 2 hours;
[0050] 3. Add 10ml of 100mM glycine to the above solution for 2 hours to block the excess active groups on the surface of latex particles;
[0051] 4. Centrifuge at 12000rpm for 20 minutes, remove the supernatant;
[0052] 5. Dilute the above-mentioned labeled CRP antibody latex particles to the working concentration with the following buffer solution, and prepare the single-reagent CRP latex enhanced immunoturbidimetric kit with calibrator. Single reagent formula: 35mM DIPSO-NaOH, pH 7.0; 0.2% Triton X-100; 0.7%NaCl; 0.5% BSA; 2% glycerol; 1% trehalose; 0.1% sodium azide.
[0053] 6. CRP calibrator: Dissolve commercially available ...
Embodiment 2
[0069] 1. Preparation of assay reagents and kits:
[0070] 1. Dilute the surface carboxylated polystyrene latex particles (150nm) with 50mM pH 6.0 MES buffer to 10ml, the final concentration is 1%;
[0071] 2. Add 200 mg of EDC to the above solution, add 10 mg of CRP antibody from affinity chromatography, and mix at room temperature for 2 hours;
[0072] 3. Add 10ml of 100mM glycine to the above solution for 2 hours to block the excess active groups on the surface of latex particles;
[0073] 4. Centrifuge at 12000rpm for 20 minutes, remove the supernatant;
[0074] 5. Dilute the above-labeled CRP antibody latex particles to the working concentration with the following buffer
[0075] Degree, together with the calibrator is the single reagent CRP latex enhanced immune turbidimetric kit. Single reagent formulation: 45mM MOPS-NaOH, pH 7.5; 0.3% Tween 20; 0.8%NaCl; 1% gelatin; 5% glycerol; 3% trehalose; 0.1% sodium azide.
[0076] 6. CRP calibrator: Dissolve commercially avai...
Embodiment 3
[0093] 1. Preparation of assay reagents and kits:
[0094] 1. Dilute the surface-aminated polystyrene latex particles (200nm) to 10ml with 50mM pH 6.0 MES buffer, the final concentration is 1%;
[0095] 2. Add 200 mg of EDC to the above solution, add 10 mg of CRP antibody from affinity chromatography, and mix at room temperature for 2 hours;
[0096] 3. Add 10ml of 100mM glycine to the above solution for 2 hours to seal the latex
[0097] Excess active groups on the particle surface;
[0098] 4. Centrifuge at 12000rpm for 20 minutes, remove the supernatant;
[0099] 5. Dilute the above-labeled CRP antibody latex particles to the working concentration with the following buffer
[0100] Degree, together with the calibrator is the single reagent CRP latex enhanced immune turbidimetric kit. Single reagent formulation: 55mM Tris-HCL, pH 8.0; 0.4% Triton X-100; 0.9% KCl; 1.5% BSA; 8% glycerol; 5% trehalose; 0.1% sodium azide.
[0101]6. CRP calibrator: Dissolve commercially ava...
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