Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Biosynthesis method of cadmium selenide quantum dots

A technology of biosynthesis and quantum dots, which is applied in the field of biosynthesis of cadmium selenide quantum dots, can solve the problems of limiting the application of quantum dots and the troubles of quantum dots, and achieves the effect of convenient operation, low cost and good biocompatibility

Inactive Publication Date: 2014-09-10
HANGZHOU NORMAL UNIVERSITY
View PDF3 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, many quantum dots have been synthesized by biological methods, but most of these quantum dots are synthesized in cells, and it is troublesome to collect the prepared quantum dots. It needs to go through processes such as cell washing, cell destruction and removal of cell debris, so it is limited. Subsequent applications of quantum dots

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Biosynthesis method of cadmium selenide quantum dots
  • Biosynthesis method of cadmium selenide quantum dots
  • Biosynthesis method of cadmium selenide quantum dots

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Escherichia coli (E.coli K12 (DH10B), provided by Beijing Dingguo Changsheng Biotechnology Co., Ltd.) was inoculated into LB medium and cultured at 37°C until OD 600 = 0.6, the seed solution was obtained, which was centrifuged, and the supernatant was removed to obtain wet cells of Escherichia coli. Take 1ml of Escherichia coli wet bacteria and inoculate it into M9 medium, and cultivate it to OD at 37°C 600 =0.6, the second-generation Escherichia coli wet cells were obtained for the preparation of quantum dots.

[0026] LB medium (500ml): 2.5g of yeast extract, 5g of peptone, 5g of sodium chloride, adjust the pH to about 7.0 with NaOH, and the solvent is water.

[0027]M9 medium (500ml): disodium hydrogen phosphate 15g, potassium dihydrogen phosphate 7.5g, ammonium chloride 2.5g, sodium chloride 1.25g, 1ml (1mol / L) magnesium sulfate heptahydrate aqueous solution, glucose 2g / L, pH Naturally, the solvent is water.

[0028] Take 0.015g of the second-generation Escherich...

Embodiment 2

[0035] Yeast (Saccharomyces cerevisiae (S288C), provided by Beijing Dingguo Changsheng Biotechnology Co., Ltd.) was inoculated into YPD medium and cultured at 37°C until OD 600 = 0.6, to obtain the seed liquid, centrifuge it, remove the supernatant, and obtain the wet yeast cell, take 1ml of the wet yeast cell and inoculate it into the Chapei medium, and continue to cultivate at 37°C until OD 600 =0.6, the second-generation yeast wet cells were obtained for the preparation of quantum dots.

[0036] YPD medium (500ml): 5g of yeast extract, 10g of peptone, 10g of glucose, adjust the pH to about 7.0 with NaOH, and the solvent is water;

[0037] Cha's medium (500ml): 15g of sucrose, 1g of sodium nitrate, 0.5g of dipotassium phosphate, 0.25g of potassium chloride, 0.25g of magnesium sulfate heptahydrate, adjust the pH to about 7.0 with NaOH, and the solvent is water.

[0038] Take 0.015g of the second-generation yeast wet thallus, transfer it into a single-necked flask, add it to ...

Embodiment 3

[0045] (1) The disposition method of Escherichia coli wet thalline is as described in embodiment 1, gets 0.0015g second generation Escherichia coli wet thalline and moves in a single-necked flask, joins the M9 substratum of 45ml (composition is the same as embodiment 1) , then add 4ml of 0.04mol·l under continuous stirring -1 CdCl 2 Aqueous solution, 400mg trisodium citrate dihydrate, 40mg MSA, 1.5ml0.01mol·l -1 Na 2 SeO 3 Aqueous solution, cultivated at 37°C for 5 days, filtered, discarded the precipitate, added an equal volume of absolute ethanol to the supernatant, centrifuged to obtain CdSe quantum dots, dried in a vacuum oven, and dried CdSe quantum dots High-resolution transmission electron microscopy imaging ( Figure 4 shown), infrared imaging ( Figure 5 shown).

[0046] (2) Escherichia coli is inoculated to the M9 medium (the culture method is the same as embodiment 1) by the wet thalli after LB medium seed culture, obtains the nutrient solution containing Esch...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
particle diameteraaaaaaaaaa
Login to View More

Abstract

The invention discloses a biosynthesis method of cadmium selenide quantum dots. The biosynthesis method comprises the following steps: by adopting selenite as an Se source, water-soluble salts of cadmium as a Cd source, a mercapto compound as a stabilizer and wet thalli obtained by carrying out fermentation culture on escherichia coli and saccharomycete as a catalyst, under the action of sodium citrate dihydrate, culturing in an escherichia coli fermentation medium or a saccharomycete fermentation medium at 37 DEG C for 1-6 days, centrifuging, discarding the supernatant, and collecting the precipitate to obtain cadmium selenide quantum dots. By virtue of the biosynthesis method disclosed by the invention, cadmium selenide quantum dots, which have adjustable and uniform size, good biocompatibility and high luminescence, are synthesized, and cadmium selenide quantum dots can be applied to dying of saccharomycete, the quantum dots show size-dependent luminescent properties, the diameter of quantum dots is about 5nm, quantum dots show a good single crystal structure under a high-resolution transmission electron microscopy and the quantum yield reaches 35%.

Description

(1) Technical field [0001] The invention relates to a synthesis method of quantum dots, in particular to a biosynthesis method of cadmium selenide quantum dots. (2) Background technology [0002] Nanotechnology is currently a hot research topic in the international field. In recent decades, it has been applied in many research directions from disease diagnosis to gene repair and targeted drug therapy, and the most concerned one is the use of quantum dots for biomarkers and dyeing etc. [0003] There are many synthesis methods of quantum dots. So far, the metal-organic method is widely used (see: Qu L, Peng Z A, et al., Nano Letters, Volume 1, 2001, 333 (2001)). and aqueous phase synthesis methods (see: Su Y, He Y, et al., Biomaterials, Vol. 30, 19(2009)). Generally speaking, chemically synthesized quantum dots have certain biological toxicity, and biomolecules must be modified on their surface to make them biocompatible before they can be applied to biomarkers. In recent ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12P3/00C12R1/19C12R1/865
Inventor 王平包海峰
Owner HANGZHOU NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com