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Preparation method of aquatic fish skin collagen hydrogel

A technology of fish skin collagen and hydrogel, which is applied in the direction of animal/human protein, connective tissue peptide, specific peptide, etc., can solve the problems of collagen application limitation, safety question, etc., and achieve safe raw material source, wide application, mechanical good strength effect

Inactive Publication Date: 2014-09-10
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the collagen used in China mainly comes from the skin and Achilles tendon of pigs and cattle, but with the occurrence of diseases such as mad cow disease and foot-and-mouth disease, people have questioned their safety; The application of collagen is limited in some regions

Method used

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  • Preparation method of aquatic fish skin collagen hydrogel
  • Preparation method of aquatic fish skin collagen hydrogel
  • Preparation method of aquatic fish skin collagen hydrogel

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1 aquatic fish skin collagen hydrogel:

[0036] 1.1 Extraction of Enzymatically Solubilized Tilapia Skin Collagen:

[0037] 1.1.1 Cleaning fish skin: Take out a certain amount of tilapia skin stored at -20°C, soak and rinse with a large amount of tap water, remove fish meat, fish scales and other tissues remaining on the surface of the fish skin, soak and wash with ultrapure water for 3 times, Then use absorbent paper to absorb the moisture on the surface of the fish skin,

[0038] 1.1.2 Degreasing: Accurately weigh 10g of the above-mentioned fish skin, cut the fish skin into 1×2cm size, and degrease it with 200mL of 10% n-butanol aqueous solution for 8 hours in a 4°C freezer under constant stirring with a magnetic stirrer , repeat the above degreasing process 3 times, then rinse with tap water for 30 minutes, and then wash with ultrapure water for 3 times;

[0039] 1.1.3 Removal of impurities: In a 4°C freezer, soak the degreased fish sk...

Embodiment 2

[0048] Embodiment 2 Determination of compressive capacity of aquatic fish skin hydrogel:

[0049] Place the hydrogel prepared under the above conditions on a horizontal glass plate, apply a vertical pressure to the hydrogel with a dynamometer with an area of ​​S, and gradually increase the pressure until the hydrogel breaks, record The instantaneous pressure F when the hydrogel is broken, calculate the anti-stress property of the collagen hydrogel product, the formula is as follows:

[0050] Compressive strength (N / cm 2 ) = F / S

[0051] The results show that the above-mentioned hydrogels with different concentrations or hydrogels with different concentrations under physiological salinity prepared by the present invention have compressive strengths in the range of 0.2-0.8, and have excellent compressive properties.

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Abstract

The invention relates to the technical field of biomedical materials and in particular relates to a preparation method of aquatic fish skin collagen hydrogel. The preparation method comprises the following steps: fully dissolving the aquatic fish skin collagen in an acetic acid solution with concentration of 0.5mol / L under the condition of continuous stirring at 4 DEG C to prepare a collagen solution; centrifuging the collagen solution after dissolution, and collecting the supernatant for later use; adjusting the pH value of the collagen supernatant with a filtered sodium hydroxide solution or mixing a filtered phosphate buffer solution with the collagen supernatant in a volume ratio of 1:1, continuously stirring the mixed solution, and then adjusting the pH value of the mixed solution with the filtered sodium hydroxide solution; putting the collagen supernatant mixed solution after the pH value is adjusted in a die, and then standing for 1-2 hours in a water bath at 30-40 DEG C so that the collagen supernatant mixed solution self aggregates, solidifies and is formed; taking the formed hydrogel out of the die, and soaking the hydrogel in ultrapure water for 20-30 minutes, thus obtaining the aquatic fish skin collagen hydrogel. The preparation method has the beneficial effects that the preparation process is simple, the materials are cheap and accessible, and the product is widely applied.

Description

technical field [0001] The invention relates to the field of biomedical material technology, in particular to a preparation method of aquatic fish skin collagen hydrogel. Background technique [0002] Collagen is the most abundant and widely distributed protein in animals. Collagen has the characteristics of unique triple helix structure, low antigenicity, non-toxicity, good biocompatibility and biodegradability, and has been widely used in tissue engineering scaffold materials, hemostatic sponges, drug controlled release systems, gene Delivery carrier, cosmetic surgery and other biomedical fields. At present, the collagen used in China mainly comes from the skin and Achilles tendon of pigs and cattle, but with the occurrence of diseases such as mad cow disease and foot-and-mouth disease, people have questioned their safety; The application of collagen has been limited in some areas. Therefore, there is an urgent need to seek collagen sources with better quality and safet...

Claims

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Application Information

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IPC IPC(8): C08J3/075C08J3/24C08L89/00C12P21/06C07K14/78C07K1/34
Inventor 闫鸣艳史文军秦松张朝晖崔红利
Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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