Method for quickly starting anaerobic ammoxidation reactor
An anammox, rapid start-up technology, applied in the field of anammox reaction, can solve the problems of slow growth rate and low metabolic rate of anammox bacteria, and achieve increased intracellular hemoglobin content, abundant sources, and stability The effect of denitrification performance
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Embodiment 1
[0030] A method for quickly starting an anammox reactor, comprising the steps of:
[0031] ① Selection of inoculum sludge: Activated sludge from Dalian Lingshui Sewage Treatment Plant was selected as the seed sludge, and the sludge concentration MLSS in the reactor was 2.54g / L.
[0032] ② Reactor: The reactor is an upflow anammox reactor made of PVC (polyvinyl chloride) material with a working volume of 0.5 liters (inner diameter 5 cm, height 25.5 cm).
[0033] ③Composition and concentration of culture medium:
[0034]
[0035] ④Reactor operating conditions: Control the internal temperature of the reactor to 35±1°C, the pH range is between 7.0 and 8.5, the dissolved oxygen is lower than 0.5mg / L, and it is protected from light.
[0036] ⑤ Reactor operation mode: adopt continuous and uninterrupted operation, and the ratio of sludge to culture solution in the reactor is 1g:10mL. During the start-up period, the hydraulic retention time (HRT) was initially set to 12 hours, the...
Embodiment 2
[0038] Others are the same as in Example 1, except that the concentration of Fe(II) is 0.06mM.
Embodiment 3
[0040] Others are the same as in Example 1, except that the concentration of Fe(II) is 0.09mM.
[0041] Table 1
[0042]
[0043]
[0044] As can be seen from Table 1, adopting this technology can accelerate the enrichment rate of anammox bacteria, shorten the start-up period of anammox reactor by about 30%, and the target bacteria obtained in Example 3 have excellent performance in all aspects. In embodiment 1 and embodiment 2. This result shows that by adjusting the concentration of Fe(II) in the culture medium, the metabolic rate of anammox bacteria can be effectively promoted, which is beneficial to the synthesis of heme c (heme c) in its cells, thereby increasing the synthesis of hydrazine Enzyme (HDH) activity, and finally realize the rapid enrichment of anammox bacteria.
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