3D printing rapidly constructed tissue engineered skin and its preparation method

Abstract

The invention discloses tissue engineering skin which comprises seed cells and an acellular allogenic dermal matrix, wherein the seed cells and the acellular allogenic dermal matrix form a skin appendage; the seed cells are arranged, spread flat and printed at corresponding positions on the front side of the acellular allogenic dermal matrix according to an anatomical structure of normal skin by using a 3D (Three-dimensional) printer. The invention also discloses a method for preparing the tissue engineering skin. The invention provides perfect tissue engineering skin, and the tissue engineering skin can be rapidly constructed in a short time in an operating room (the operation is finished). Meanwhile, the tissue engineering skin comprises the seed cells and dermal matrix, the seed cells are basal layer cells, have the activities of multipotential stem cells and multipotential differentiation functions and are arranged and printed in the corresponding positions according to the anatomical structure of the normal skin, the skin appendage is easily formed, the cure rate is accelerated after the operation, and functions of the skin are restored.

Description

technical field [0001] The invention relates to the technical field of tissue engineering skin, and also relates to a construction method of tissue engineering skin. Background technique [0002] Wound is the loss of skin integrity due to internal or external factors. Its repair has always been the main problem in clinical practice. Clinically, it can be divided into acute wound and chronic wound according to time. Acute wounds include burns, trauma and other major diseases, or skin defects formed after surgical resection of scars, skin pigmented diseases, benign and malignant tumors, etc.; chronic wounds include traumatic ulcers, diabetic ulcers, vascular ulcers, pressure ulcers, radiation Ulcers, tumorous ulcers, cicatricial ulcers, etc. In recent years, the incidence of chronic wounds has been increasing year by year. The disease course is long, difficult to heal, the survival rate of skin grafts is low, and the skin function is lost after skin grafting, resulting in a d...

AI Technical Summary

Problems solved by technology

[0003] However, whether it is traditional skin grafting, skin flap transplantation, stamp skin grafting, micro-skin grafting, reticular skin grafting, composite skin grafting, etc., or the emerging tissue engineered skin (referring to the combination of dermal fibroblasts and extracellular matrix Alternatives are mixed to make artificial skin, or simply use porous extracellular matrix instead of dermis to implant the wound, and then transplant epithelial cells to cover it or wait for its own epithelial cover to replace the damaged skin), so far, it has not effectively solved the problem of wound repair

Skin flaps, skin flaps, stamp skins, particle skins, mesh skins, composite skins and other skins used in the above surgical methods all have the following defects after surgery: scar hyperplasia, loss of sweat glands, and loss of original skin after recovery from burn patients with serious diseases. patients with chronic wounds will not heal.