High-efficiency and rapid micropropagation method for toxicodendron vernicifluum
A small wood paint, fast technology, applied in horticultural methods, botanical equipment and methods, climate change adaptation, etc., can solve the problems of low seedling formation rate and slow reproduction speed, and achieve stable genetic traits, easy rooting, and cultivation process. simple effect
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[0016] Example 1. The implementation time was 2013. The tissue culture and propagation of small wood lacquer was carried out in the laboratory of the College of Forestry of Southwest Forestry University and the Greenhouse.
[0017] (1) Selection and disinfection of explants: In May 2013, select a good sumac single plant to obtain its tender stems of the year, remove the petioles and leaves, brush the stem surface with a brush to remove surface impurities, and use 2% Soak in the detergent aqueous solution for 10 minutes, rinse in linear tap water for 2 hours, cut into 3-4cm long stems with 1-2 leaf axils, put them in the sterilized explant tank, and move to ultra-clean work Disinfect inside the station, soak in 75% ethanol for 20 seconds, soak in 1% benzalkonium chloride solution for 1 minute, rinse in sterile distilled water for 2-3 times, soak in 0.1% mercury liters for 2 minutes, and rinse in sterile distilled water for 5-8 Second, put it in the sterilized inoculation tray with...
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