Feed microecological preparation and preparation method thereof
A microecological preparation and feeding technology, which is applied in the field of feeding microecological preparations and its preparation, can solve the problems of poor stability and repeatability of the effect, and ineffective application effects, etc., and achieve strong palatability, increase daily weight gain, and improve quality effect
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Embodiment 1
[0020] Embodiment 1 kind of liquid preparation:
[0021] 1. Source of bacteria
[0022] Lactobacillus acidophilus source: CGMCC, Institute of Microbiology, Chinese Academy of Sciences, Lactobacillus plantarum subspecies, AS1.2707; Bacillus subtilis source: CGMCC, Institute of Microbiology, Chinese Academy of Sciences, Bacillus subtilis Subspecies, AS1.2163; Saccharomyces cerevisiae source: CGMCC, Culture Collection Center, Institute of Microbiology, Chinese Academy of Sciences, Saccharomyces cerevisiae, AS2.3875.
[0023] 2. Saccharomyces cerevisiae
[0024] 2.1 Incline strains: medium: yeast extract 1wt%, peptone 2wt%, glucose 2wt%, agar 2wt%. Sterilize at 0.1Mpa for 20 minutes, inoculate aseptically, and incubate at 28°C for 48 hours.
[0025] 2.2 Erlenmeyer flask shaker culture: medium: glucose 2wt%, urea 0.5wt%, ammonium sulfate 1wt%, yeast extract 0.5wt%. Sterilize at 0.1Mpa for 20 minutes, inoculate aseptically, and culture on a shaker at 28°C for 48 hours, with...
Embodiment 2
[0064] The preparation method of the present embodiment feed probiotics comprises the following steps:
[0065] (1) Strain equipment: equipped with Lactobacillus acidophilus 32.1х10 8 cfu / L, Saccharomyces cerevisiae 1х10 8 cfu / L, Bacillus subtilis 61.5х10 8 cfu / L;
[0066] (2) Preparation of solid medium: by weight percentage, mix the following raw materials evenly: bran 80%, apple pomace 15%, corn flour 2%, brown sugar 2%, dipotassium hydrogen phosphate 0.5% and ammonium sulfate 0.5%; After sterilization, add water to adjust the water content to 12wt%, and pack it into solid material bags (plastic bags with one-way air valves that ventilate outward);
[0067] (3) Inoculation and fermentation: first mix Lactobacillus acidophilus and Saccharomyces cerevisiae at a volume ratio of 1:1, then inoculate the solid medium at an inoculum amount of 30wt% relative to the solid medium, and incubate at 30°C After 7 days of anaerobic fermentation, the inoculation amount of 10wt% rela...
Embodiment 3
[0069] The preparation method of the present embodiment feed probiotics comprises the following steps:
[0070] (1) Strain equipment: equipped with Lactobacillus acidophilus 3х10 9 cfu / L, Saccharomyces cerevisiae not less than 1.1х10 8 cfu / L, Bacillus subtilis not less than 6х10 9 cfu / L;
[0071] (2) Preparation of solid medium: by weight percentage, mix the following raw materials evenly: bran 75%, apple pomace 20%, corn flour 1%, brown sugar 3%, dipotassium hydrogen phosphate 0.2% and ammonium sulfate 0.8%; After sterilization, add water to adjust the water content to 10wt%, and pack it into solid material bags (plastic bags with one-way air valves that ventilate outward);
[0072] (3) Inoculation and fermentation: first mix Lactobacillus acidophilus and Saccharomyces cerevisiae at a volume ratio of 1:0.8, and then inoculate the above solid medium at an inoculation amount of 25wt% relative to the solid medium, at 25°C After 8 days of anaerobic fermentation, the inocul...
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