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Method for reducing background signal of electrochemical sensor and sensor utilizing method

A background signal and sensor technology, which is applied in the field of biological analysis, can solve the problems that the background signal cannot be effectively reduced, and achieve the effect of improving sensitivity, reducing background signal, and improving relative signal change

Active Publication Date: 2014-08-13
CAPITAL NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in many cases these methods are not effective in reducing the background signal

Method used

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  • Method for reducing background signal of electrochemical sensor and sensor utilizing method
  • Method for reducing background signal of electrochemical sensor and sensor utilizing method
  • Method for reducing background signal of electrochemical sensor and sensor utilizing method

Examples

Experimental program
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Effect test

Embodiment l

[0034] Example 1: Using electrochemical impedance spectroscopy to confirm the non-specific adsorption of molecular probes on the surface of gold electrodes, and to study the relationship between the height and density of DNA self-assembled monolayers on the electrode surface and the strength of non-specific adsorption.

[0035] When preparing DNA self-assembly layers of different heights, respectively reduce 1 μM probes 1, 2 and 3 in 100 μM tris[2-carboxyethyl]phosphine (TCEP), 10 mM PB, 1.0 M NaCl, pH=7.0 at room temperature for 1 h , and then soak unmodified clean gold electrodes into it, and self-assemble overnight at room temperature. The assembled electrode was rinsed three times with ultrapure water, then placed in an aqueous solution containing 1mMMCH to seal for 30 minutes, and then rinsed three times with ultrapure water; when preparing self-assembled layers with different densities, 1 μM probe 1 Reduction in 100μM TCEP, 10mM PB, 0.2M NaCl, pH=7.0; 100μM TCEP, 10mM PB...

Embodiment 2

[0038] Example 2: Immobilizing molecular signaling probes labeled with electrochemical redox groups to the surface of gold nanoparticles to synthesize nanoprobes.

[0039] The molecular signal probe labeled with the electrochemical redox group was dissolved in an aqueous solution of 50 mM dithiothreitol (DTT) and 2% triethylamine (TEA) by volume, and reacted at room temperature for 20 min. The NAP-5 column was used to purify the column twice, and the purified molecular signal probe was quantified by the UV-visible absorption value at 260nm to the reduced molecular signal probe. Then it was added to 9nM nano-gold solution with a particle size of 13nm, the molar ratio of molecular signal probes to nano-gold was 100:1, and aged at room temperature for 16 hours. After aging, add a solution of high salt concentration (10mM PB, 1M NaCl, pH7.4) several times under shaking in the molecular hybridization instrument to make the final concentration of NaCl in the system 0.2M. After shaki...

Embodiment 3

[0040] Example 3: Using nano-probes to remove non-specific adsorption on the surface of sensor electrodes to improve the sensitivity of the signal-increasing detection platform cocaine electrochemical sensor.

[0041] 1 μM probe 5 was reduced in 100 μM TCEP, 10 mM PB, 1.0 M NaCl, pH=7.0 at room temperature for 1 h, and then an unmodified clean gold electrode was soaked in it, and self-assembled overnight at room temperature. The assembled electrode was rinsed three times with ultrapure water, then placed in an aqueous solution containing 1 mM mercaptohexanol (MCH) to seal the electrode for 30 minutes, and then rinsed three times with ultrapure water. The needles were dissolved in a buffer solution (10 mM PB, 0.2 M NaCl, pH 7.0), and the final concentration of gold nanoparticles was 5 nM. A three-electrode system was composed of the above-mentioned gold electrode assembled with probe 5 as the working electrode, and a certain concentration of cocaine was added to the electrolyte...

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Abstract

The invention relates to a method for reducing a background signal of an electrochemical sensor and a sensor utilizing the method, which belong to the technical field of biological analysis. The method is characterized in that a conventional lotus effect in the nature is formed in the establishment or detection process of a sensor, a molecular signal probe in small size is fixed on the surface of nanogold particles to be synthesized into a nano probe in a large size, so that a nanostructure which is similar to the surface of a lotus leaf is formed in the sensing process, the adsorption caused by the insertion of the signal probe into a molecular gap on a DNA single molecular layer on the surface of an electrode can be effectively prevented, and the background signal of the electrochemical sensor can be basically eliminated. A result shows that the background signals can be completely eliminated by adopting the nano probe when cocaine and triphosadenine are detected, the sensitivity in the detection can be correspondently and greatly improved, and the detection sensitivity of the cocaine and triphosadenine are respectively 6nm and 5nm and respectively improved by 2000 times and 1000 times; when Hg2+ is detected, the signal residue is also reduced from 30 percent to be less than 6 percent.

Description

technical field [0001] The invention relates to a method for reducing the background signal of an electrochemical sensor and an application thereof, belonging to the technical field of biological analysis. Background technique [0002] Sensors based on solid-phase substrates, such as microarrays, test strips, planar waveguide systems and electrodes, are widely used in basic research and practice due to their advantages of high throughput, simple measurement, portability and automation, and good miniaturization compatibility. detection. However, such sensors also have many technical difficulties that limit their applications. The most common and serious problem is that the non-specific adsorption of the signal probe on the surface of the solid phase matrix causes a strong background signal, which leads to a decrease in the detection sensitivity of the sensor. Self-assembled molecular layer passivation surface (Sens Actuators B Chem.2008, 129(1), 225-230.) polymer (Anal.Chem...

Claims

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Application Information

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IPC IPC(8): G01N27/416
Inventor 娄新徽赵滔刘然何苗
Owner CAPITAL NORMAL UNIVERSITY
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