Fabrication method for hydrogel/macromolecule polymer film muscular tissue support

Abstract

The invention discloses a fabrication method for a hydrogel / macromolecule polymer film muscular tissue support. The method comprises obtaining a three-dimensional support structure through a layer-upon-layer stacking mode; designing and fabricating a blood vessel network structure mold and a directional flow passage mold according to muscle structural characteristics; fabricating polymer directional film through an electro-spinning technology, fabricating a support single-layer plane structure, stacking other layers of supports on the three-dimensional support structure, and performing covering with the macromolecule polymer film support bonded on the outermost layer to obtain the hydrogel / macromolecule polymer film support. According to the method, the blood vessel network and the directional flow passage are fabricated on hydrogel, directional growth of cells can be achieved through the directional flow passage, nutrition and oxygen supply can be achieved through blood vessel network pipes, the polymer film covered on the periphery has a directional structure, and accordingly, directional growth of peripheral cells can be achieved, stretched film is bound and sewn, and fixation of the support is achieved.

Description

technical field [0001] The invention relates to a method for preparing a muscle tissue scaffold, in particular to a method for manufacturing a hydrogel / high polymer film muscle tissue scaffold. Background technique [0002] Muscle includes cardiac muscle, smooth muscle, and skeletal muscle. The three have myofibrils, blood vessels, and nerves in common, and the muscle cells are arranged in a directional arrangement. The difference lies in the distribution and shape of the three tissues, the number, shape and function of myofibrils. Skeletal muscle is a relatively large soft tissue in the human body, accounting for 40% to 45% of the body weight. It is connected to the bone through tendons. When receiving stimulation, the contraction and relaxation of skeletal muscle realizes the function of body movement. Normal muscle regeneration is mainly completed by muscle satellite cells (satellite cells, SCs), but the number of SCs is small and decreases with age. For severe or large...

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Problems solved by technology

For severe or large-scale muscle injuries, SC is not enough to achieve the purpose of repair, and the wound surface will eventually be replaced by fibrous tissue to form scars, resulting in difficult wound healing and loss of motor function.

At present, for large-scale muscle damage, the most common clinical treatment is myocutaneous flap transplantation. This method causes great damage to the donor area and the source is limited. Muscle tissue scaffolds provide the possibility to overcome these shortcomings, but the existing muscle tissue The preparation process of the scaffold is relatively difficult, the three-dimensional construction of the muscle tissue scaffold with vascular structure is difficult, and the fixation of the muscle tissue scaffold is inconvenient

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