Sex pheromone composition of orthaga olivacea warre and lure core thereof
A technology of sex and pheromone of C. sylvestris, which is applied in the fields of pest control, animal repellent, and pest-attracting agent, etc., can solve the problem of no papers and patents reporting the sex pheromone of C. sylvestris
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[0043] The present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments.
[0044] 1. Extraction of sex pheromones
[0045]Collect aged or mature larvae, raise them in the laboratory, and prepare them after eclosion; use sexually mature unmated female moths, aged 2-3 days after eclosion, and extract them 5-6 hours after dark at night. Take adult moths, after ether anesthesia, press the abdomen lightly with medical surgical tweezers, then cut off the 3 segments at the end of the abdomen, transfer them into a clean pointed-bottomed glass vial filled with about 100 μl double-distilled n-hexane, soak for 10 minutes, and draw Part of the solution was transferred to a sharp-bottomed glass tube, concentrated under slight nitrogen, and analyzed by gas phase-mass spectrometry.
[0046] 2. Identification
[0047] Gas phase analysis conditions: All synthetic sex pheromone compounds are analyzed on Agilent6890GC-5975MS, the chr...
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