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Real-time dynamic detection system for superoxide radicals produced by photocatalytic reaction

A real-time dynamic, detection system technology, applied in the direction of chemical reaction of materials for analysis, chemiluminescence/bioluminescence, etc., to achieve the effects of easy commercial promotion, improved work efficiency, and simple operation

Active Publication Date: 2016-06-15
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, no flow chemiluminescence method has been used to measure the O produced by photocatalytic reactions at home and abroad. 2 ·- report

Method used

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  • Real-time dynamic detection system for superoxide radicals produced by photocatalytic reaction
  • Real-time dynamic detection system for superoxide radicals produced by photocatalytic reaction
  • Real-time dynamic detection system for superoxide radicals produced by photocatalytic reaction

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Embodiment Construction

[0029] The experimental methods used in the following examples are conventional methods unless otherwise specified.

[0030] The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

[0031] Such as figure 1 As shown in (a), the real-time dynamic detection system for superoxide free radicals produced by photocatalytic reaction provided by the present invention includes an excitation light source 1, a photocatalyst holding container 2, a chemiluminescent probe holding container 4, a detection cell 5, a photoelectric Multiplier tube 6 and free radical quencher holding container 3 . The excitation light source 1 can be a mercury lamp or a xenon lamp, which is used to excite photocatalysts or photosensitive molecules. The photocatalyst holding container 2 is used to hold the photocatalyst or photosensitive molecule to be detected, the photocatalyst holding container 2 is connected with the detection cell 5, and...

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Abstract

The invention discloses a real-time dynamic detection system for generating superoxide radical through a light-catalyzed reaction. The system comprises an excitation light source, a photocatalyst containing container, a chemiluminescence probe containing container, a detection cell, a photomultiplier and a signal analyzer host, wherein both the photocatalyst containing container and the chemiluminescence probe containing container are communicated with the detection cell; the photomultiplier is used for converting an optical signal generated in the detection cell into an electric signal; the photomultiplier is connected with the signal analyzer host; the signal analyzer host is connected with a computer terminal. When the real-time dynamic detection system is used for detecting, online measurement can be performed, the operation is simple, and a sample does not need to be subjected to processes such as offline separation and computer detection. Therefore, the measurement speed is high, the working efficiency is improved, and the system is suitable for rapidly screening and evaluating the capability of generating superoxide radical in various photocatalytic materials and photosensitive molecules. According to the system, online real-time measurement can be realized, dynamic change of superoxide radical can be traced on line, and the generation and attenuation behavior of superoxide radical can be researched through dynamic information.

Description

technical field [0001] The invention relates to a real-time dynamic detection system for superoxide free radicals produced by photocatalytic reactions. Background technique [0002] Superoxide radicals (O 2 ·- ) is an important class of reactive oxygen species that widely exist in organisms and environmental media. In organisms, O 2 ·- It is an important small molecular substance that participates in a series of physiological and biochemical processes, such as immune response, signal transduction and oxidative stress. In ambient media, the current O 2 ·- The environmental behaviors such as the migration and transformation of O 2 ·- Mediates many redox reactions in environmental media. As confirmed by the latest research, in the environmental water body, dissolved organic matter (such as humic acid, etc.) undergoes a photosensitization reaction under light to reduce dissolved oxygen to generate O 2 ·- , O 2 ·- Able to restore Ag in water + Generate nano-Ag. The...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/76
Inventor 赵利霞王大彬郭良宏耿方兰
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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