Goat MSTN (myostatin) gene fixed-point modification system and application thereof
A site-directed modification, gene technology, applied in the field of genetic engineering, can solve the problems of small fragment base loss or insertion, affecting gene function, etc.
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[0036] The present invention is described in further detail below in conjunction with accompanying drawing and specific implementation case, but not as the limitation of the present invention, and concrete operation process is carried out according to the following steps:
[0037] The first step is to design a specific sequence TALEN target according to the gene sequence to be knocked out;
[0038] The second step is to construct a TALEN plasmid according to the designed TALEN target;
[0039] Step 3: Transfect recipient cells with TALEN plasmids;
[0040] The fourth step is to screen, cultivate, and pick monoclonal cells after the transformation, expand the culture of monoclonal cells, identify the genomic mutation of the monoclonal cells, and select positive cell clones that meet the mutation requirements for subsequent experiments.
[0041] The experimental methods used in the following examples are conventional methods unless otherwise specified.
[0042] Materials, reag...
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