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A dipstick used for testing sulfonamides and fluoroquinolones and test method thereof

A technology for sulfonamides and fluoroquinolones, which is applied in the field of test strips for detecting sulfonamides and fluoroquinolones, can solve the problem that the method for simultaneous detection of multiple antibiotics is less researched, and is not suitable for large-scale sample screening and on-site The detection and processing process is complicated, and the detection time is short, easy to popularize and use, and the operation is simple.

Active Publication Date: 2014-07-23
BEIJING KWINBON BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the large difference in the properties of veterinary drugs, the existing residue analysis generally establishes corresponding detection methods according to the same family of drugs with similar chemical structures. There are few researches on the method, which cannot meet the needs of the increasing variety of veterinary drugs and the continuous improvement of the analysis throughput.
At present, there are also researches on liquid chromatography-mass spectrometry technology. This method has high sensitivity, good selectivity and specificity, and can accurately confirm the qualitative confirmation of low-concentration samples. However, the sample pretreatment process is complicated and the degree of instrumentation is high. And the price is expensive, the analysis speed is slow, it is only suitable for the confirmation analysis of samples, and it is not suitable for the screening and on-site detection of large quantities of samples

Method used

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  • A dipstick used for testing sulfonamides and fluoroquinolones and test method thereof
  • A dipstick used for testing sulfonamides and fluoroquinolones and test method thereof
  • A dipstick used for testing sulfonamides and fluoroquinolones and test method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0043] Example 1 The composition of test strips for detecting sulfa drugs and fluoroquinolones

[0044] 1. Test paper ( figure 1 )

[0045] The test paper is composed of a bottom plate, a sample absorption pad, a reaction film, a water absorption pad, and a protective film;

[0046] The sample absorbent pad 1, the reaction film 2, the absorbent pad 3 and the protective film 7 are sequentially pasted on the bottom plate 6 in order. The end of the sample absorbent pad is connected to the reaction membrane, the end of the reaction membrane is connected to the absorbent pad, and the The beginning end is aligned with the beginning end of the bottom plate, and the end of the absorbent pad is aligned with the end of the bottom plate;

[0047] The test paper is pasted with a protective film, and the protective film 7 covers the sample absorption pad, which is the detection end, and the word MAX is printed on it ( figure 2 );

[0048] The reaction membrane has a detection line 4-1, a detectio...

Embodiment 2

[0053] Example 2 The preparation method of the test strip described in Example 1

[0054] 1. Preparation of test strips

[0055] The preparation method of the test strip mainly includes the following steps:

[0056] 1) Preparation of freeze-dried microporous reagents with sulfa drugs monoclonal antibody-colloidal gold marker and fluoroquinolone drug monoclonal antibodies-colloidal gold marker;

[0057] 2) Prepare two test lines coated with sulfa drug hapten-carrier protein conjugate and fluoroquinolone drug hapten-carrier protein conjugate and a quality control line coated with goat anti-mouse anti-antibody The reaction film;

[0058] 3) Assemble the prepared reaction membrane in 2) with sample absorbent pad, absorbent pad, protective film, and bottom plate into test paper;

[0059] 4) Assemble the prepared microporous reagents and test strips containing sulfa drug monoclonal antibody-colloidal gold marker and fluoroquinolone drug monoclonal antibody-colloidal gold marker prepared in 1)...

Embodiment 3

[0111] Example 3 Detection of sulfa drugs and fluoroquinolones in milk

[0112] 1. Test with test strips

[0113] Take out the required number of microporous reagents and test papers from the original packaging (if stored at low temperature, pre-equilibrate to room temperature), and mark them; use a micropipette to draw 200μl of the milk solution to be tested into the microwells, slowly aspirate and Mix well with the reagents in the microwell; insert the marked test paper into the microwell-the end of the line marked with "MAX" is facing down, so that it is fully immersed in the solution; incubate at room temperature (20-25°C) for 5 minutes, and take out the test paper. judgement result.

[0114] 3. Analysis of test results

[0115] Negative: When the quality control line (C) shows a red band, the test line (T 1 ) And detection line (T 2 ) At the same time, it also shows a red band, which is judged as negative, such as Figure 4 shown in a;

[0116] Sulfonamide drugs are positive: when...

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Abstract

The invention discloses a dipstick used for testing sulfonamides and fluoroquinolones and test method thereof. The dipstick consists of a dipstick body and a microporous reagent, the said microporous reagent contains a freeze-dried sulfonamides monoclonal antibody-colloidal gold marker and a freeze-dried fluoroquinolones monoclonal antibody-colloidal gold marker; the said dipstick body consists of a sample absorption pad, a reaction film, an absorbent pad, a protective film and a bottom patch which are connected in sequence, the said reaction film includes two detection lines and a quality control line, the said two detection lines are respectively coated with sulfonamides hapten carrier protein conjugates and fluoroquinolones hapten carrier protein conjugates, the said quality control line is coated with goat-anti-mouse antibody. Using the dipstick introduced in this invention to test sulfonamides and fluoroquinolones is convenient, fast, intuitive, and accurate. The dipstick is convenient to carry, is wide in application range and low in cost and is easy to promote and use.

Description

Technical field [0001] The invention relates to a test strip and a method for detecting sulfa drugs and fluoroquinolone drugs, in particular to a colloidal gold test strip for simultaneously detecting sulfa drugs and fluoroquinolone drugs in milk. technical background [0002] Sulfonamides (Sulfonamides, SAs) are a class of chemotherapeutic drugs with a p-aminobenzenesulfonamide structure, which inhibit the reproduction of bacteria by affecting the synthesis of nuclear proteins. Fluoroquinolones (FQs) are synthetic antibacterial drugs containing the basic structure of 4-quinolone, which achieve antibacterial effects by inhibiting the synthesis of bacterial DNA. Because FQs and SAs can inhibit most Gram-positive and negative bacteria, they are stable in nature, low in price, and convenient to use. Sub-therapeutic concentrations of drugs are often used as feed additives to prevent the occurrence of diseases, increase the conversion rate of feed, and promote animals Grow. However,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/04
CPCG01N33/558G01N33/9446
Inventor 何方洋冯才伟田甜冯月君崔海峰余厚美徐念琴
Owner BEIJING KWINBON BIOTECH
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